POLR2J2 Antibody (OTI3G4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03649
![Flow Cytometry: POLR2J2 Antibody (OTI3G4) [NBP2-03649]](https://resources.bio-techne.com/images/products/POLR2J2-Antibody-3G4-Flow-Cytometry-NBP2-03649-img0001.jpg "Flow Cytometry: POLR2J2 Antibody (OTI3G4) [NBP2-03649]")
![Western Blot: POLR2J2 Antibody (OTI3G4) [NBP2-03649]](https://resources.bio-techne.com/images/products/POLR2J2-Antibody-3G4-Western-Blot-NBP2-03649-img0002.jpg "Western Blot: POLR2J2 Antibody (OTI3G4) [NBP2-03649]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI3G4
Concentration
0.72 mg/ml
Product Summary for POLR2J2 Antibody (OTI3G4)
Immunogen
Full length human recombinant protein of human POLR2J2 (NP_116581) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
12.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for POLR2J2 Antibody (OTI3G4)
Western Blot: POLR2J2 Antibody (OTI3G4) [NBP2-03649]
Western Blot: POLR2J2 Antibody (3G4) [NBP2-03649] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
Immunocytochemistry/Immunofluorescence: POLR2J2 Antibody (3G4) [NBP2-03649] Staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2J2.
![Immunohistochemistry-Paraffin: POLR2J2 Antibody (OTI3G4) [NBP2-03649]](https://resources.bio-techne.com/images/products/POLR2J2-Antibody-3G4-Immunohistochemistry-Paraffin-NBP2-03649-img0003.jpg "Immunohistochemistry-Paraffin: POLR2J2 Antibody (OTI3G4) [NBP2-03649]")
Immunohistochemistry-Paraffin: POLR2J2 Antibody (OTI3G4) [NBP2-03649]
Immunohistochemistry-Paraffin: POLR2J2 Antibody (3G4) [NBP2-03649] - Staining of paraffin-embedded Human Kidney tissue using anti-POLR2J2 mouse monoclonal antibody.Applications for POLR2J2 Antibody (OTI3G4)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.72 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: POLR2J2
Alternate Names
DNA directed RNA polymerase II polypeptide J-related, DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, MGC105050, MGC54043, polymerase (RNA) II (DNA directed) polypeptide J2, RNA polymerase II subunit B11-b1, RPB11, RPB11b1DNA-directed RNA polymerase II subunit J2
Entrez Gene IDs
246721 (Human)
Gene Symbol
POLR2J2
Additional POLR2J2 Products
Product Documents for POLR2J2 Antibody (OTI3G4)
Product Specific Notices for POLR2J2 Antibody (OTI3G4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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