POLR2J2 Antibody (OTI3G4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03649

Novus Biologicals, part of Bio-Techne
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NBP2-03649
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3G4

Concentration

0.72 mg/ml

View all POLR2J2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human POLR2J2 (NP_116581) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

12.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POLR2J2 Antibody (OTI3G4)

Western Blot: POLR2J2 Antibody (OTI3G4) [NBP2-03649]

Western Blot: POLR2J2 Antibody (OTI3G4) [NBP2-03649]

Western Blot: POLR2J2 Antibody (3G4) [NBP2-03649] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
Immunocytochemistry/Immunofluorescence: POLR2J2 Antibody (3G4) [NBP2-03649] Staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2J2.
Immunohistochemistry-Paraffin: POLR2J2 Antibody (OTI3G4) [NBP2-03649]

Immunohistochemistry-Paraffin: POLR2J2 Antibody (OTI3G4) [NBP2-03649]

Immunohistochemistry-Paraffin: POLR2J2 Antibody (3G4) [NBP2-03649] - Staining of paraffin-embedded Human Kidney tissue using anti-POLR2J2 mouse monoclonal antibody.
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Applications for POLR2J2 Antibody (OTI3G4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.72 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLR2J2

This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq].

Alternate Names

DNA directed RNA polymerase II polypeptide J-related, DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, MGC105050, MGC54043, polymerase (RNA) II (DNA directed) polypeptide J2, RNA polymerase II subunit B11-b1, RPB11, RPB11b1DNA-directed RNA polymerase II subunit J2

Entrez Gene IDs

246721 (Human)

Gene Symbol

POLR2J2

Additional POLR2J2 Products

Product Documents for POLR2J2 Antibody (OTI3G4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for POLR2J2 Antibody (OTI3G4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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