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POLR2J2 Antibody (OTI3G4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73520

Novus Biologicals, part of Bio-Techne
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NBP2-73520

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3G4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human POLR2J2 (NP_116581) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

12.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for POLR2J2 Antibody (OTI3G4) - Azide and BSA Free

Western Blot: POLR2J2 Antibody (OTI3G4)Azide and BSA Free [NBP2-73520]

Western Blot: POLR2J2 Antibody (OTI3G4)Azide and BSA Free [NBP2-73520]

Western Blot: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.
Immunocytochemistry/ Immunofluorescence: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]

Immunocytochemistry/ Immunofluorescence: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]

Immunocytochemistry/Immunofluorescence: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2J2.
Immunohistochemistry: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]

Immunohistochemistry: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]

Immunohistochemistry: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520] - Staining of paraffin-embedded Human Kidney tissue using anti-POLR2J2 mouse monoclonal antibody.

Applications for POLR2J2 Antibody (OTI3G4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: POLR2J2

The POLR2J2 gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has

Alternate Names

DNA directed RNA polymerase II polypeptide J-related, DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, MGC105050, MGC54043, polymerase (RNA) II (DNA directed) polypeptide J2, RNA polymerase II subunit B11-b1, RPB11, RPB11b1DNA-directed RNA polymerase II subunit J2

Gene Symbol

POLR2J2

Additional POLR2J2 Products

Product Documents for POLR2J2 Antibody (OTI3G4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for POLR2J2 Antibody (OTI3G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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