POLR2J2 Antibody (OTI3G4) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73520
![Flow Cytometry: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]](https://resources.bio-techne.com/images/products/POLR2J2-Antibody-OTI3G4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73520-img0002.jpg "Flow Cytometry: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]")
![Western Blot: POLR2J2 Antibody (OTI3G4)Azide and BSA Free [NBP2-73520]](https://resources.bio-techne.com/images/products/POLR2J2-Antibody-OTI3G4-Azide-and-BSA-Free-Western-Blot-NBP2-73520-img0001.jpg "Western Blot: POLR2J2 Antibody (OTI3G4)Azide and BSA Free [NBP2-73520]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI3G4
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human POLR2J2 (NP_116581) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
12.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for POLR2J2 Antibody (OTI3G4) - Azide and BSA Free
Western Blot: POLR2J2 Antibody (OTI3G4)Azide and BSA Free [NBP2-73520]
Western Blot: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2.![Immunocytochemistry/ Immunofluorescence: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]](https://resources.bio-techne.com/images/products/POLR2J2-Antibody-OTI3G4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73520-img0003.jpg "Immunocytochemistry/ Immunofluorescence: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]")
Immunocytochemistry/ Immunofluorescence: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]
Immunocytochemistry/Immunofluorescence: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2J2.![Immunohistochemistry: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]](https://resources.bio-techne.com/images/products/POLR2J2-Antibody-OTI3G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73520-img0004.jpg "Immunohistochemistry: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]")
Immunohistochemistry: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520]
Immunohistochemistry: POLR2J2 Antibody (OTI3G4) - Azide and BSA Free [NBP2-73520] - Staining of paraffin-embedded Human Kidney tissue using anti-POLR2J2 mouse monoclonal antibody.Applications for POLR2J2 Antibody (OTI3G4) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: POLR2J2
Alternate Names
DNA directed RNA polymerase II polypeptide J-related, DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, MGC105050, MGC54043, polymerase (RNA) II (DNA directed) polypeptide J2, RNA polymerase II subunit B11-b1, RPB11, RPB11b1DNA-directed RNA polymerase II subunit J2
Gene Symbol
POLR2J2
Additional POLR2J2 Products
Product Documents for POLR2J2 Antibody (OTI3G4) - Azide and BSA Free
Product Specific Notices for POLR2J2 Antibody (OTI3G4) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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