Polyoma Virus, Medium T Antigen Antibody (PyMT)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2749

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Virus

Cited:

Mouse, Virus

Applications

Validated:

Dual RNAscope ISH-IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Dual RNAscope ISH-IHC, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # PyMT

Concentration

1.0 mg/ml

View all Polyoma Virus, Medium T Antigen Antibodies »

Product Specifications

Immunogen

Polyoma Virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.

Reactivity Notes

Polyoma Virus. Mouse reactivity reported in multiple pieces of scientific literature.

Localization

Cytoplasmic location in cells infected with virus.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Polyoma Virus, Medium T Antigen Antibody (PyMT)

Immunocytochemistry/ Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749]

Immunocytochemistry/ Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749]

Immunocytochemistry/Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - PyMT-transgenic mouse mammary glands were stained in green for the PyMT protein. Staining showed good membrane/cytoplasmic localization with moderate signal for most cells in mammary tumor. Blocking performed with 20% goat serum. Image from verified customer review.
Immunohistochemistry-Paraffin: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749]

Immunohistochemistry-Paraffin: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749]

Immunohistochemistry-Paraffin: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - Darpp-32 and t-Darpp expression inPpp1r1bknockout mice. Mammary tumor samples were formalin-fixed and serial sections were stained for Darpp-32 and PyMT (5x magnification in a 6 x 6 tile, scale bar = 2000 um; images enlarged from the boxed regions are at 10x magnification, scale bar = 200 um). Image collected and cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-192), licensed under a CC-BY license.
Immunohistochemistry: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749]

Immunohistochemistry: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749]

Immunohistochemistry: Polyoma Virus, Medium T Antigen Antibody (PyMT) [NB100-2749] - AREG not expressed in PyMT lesions. Representative images of PyMT immunofluorescent staining of 6-week-old AREG +/+ PyMT and AREG -/- PyMT MFPs, respectively. Scale bar shows 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30367629/) licensed under a CC-BY license.
View 2 more images

Applications for Polyoma Virus, Medium T Antigen Antibody (PyMT)

Application
Recommended Usage

Dual RNAscope ISH-IHC

reported in scientific literature (PMID 30367629)

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 19816927)

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Reviewed Applications

Read 1 review rated 4 using NB100-2749 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Polyoma Virus, Medium T Antigen

Polyoma Viruses are a class of potentially oncogenic DNA viruses that often persist as latent infections in a host without causing disease, but may produce tumors in a host of a different species. Of particular interest, is that only the rodent Polyoma Viruses code for the production of PyMT (Medium T Antigen). This oncogenic protein of the Polyoma Virus is associated with cell membranes and interacts with a variety of cellular proteins involved in mitogenic signaling, such as tyrosine kinases c-Src, c-Yes, and Fyn. Medium T Antigen may therefore mimic the function of cellular tyrosine kinase growth factor receptors, like the platelet-derived growth factor or epidermal growth factor receptor (PMCID: PMC191984). Furthermore, it has been found that the Medium T Antigen may have a mechanism for inactivating p53, as it seems to be a characteristic of oncogenic DNA viruses (PMID: 22261839).

Alternate Names

Middle t antigen, MPyVgp2, Polyoma virus middle T antigen, Polyomavirus medium t antigen, Polyomavirus middle t antigen

Additional Polyoma Virus, Medium T Antigen Products

Product Documents for Polyoma Virus, Medium T Antigen Antibody (PyMT)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Polyoma Virus, Medium T Antigen Antibody (PyMT)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.