Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80918

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Virus

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # PyMT

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Polyoma Virus, Medium T Antigen Antibodies »

Product Specifications

Immunogen

Polyoma Virus-transformed Wistar rat fibroblast cell line Py REWA5/T1A1.

Reactivity Notes

Polyoma Virus. Mouse reactivity reported in multiple pieces of scientific literature.

Localization

Cytoplasmic location in cells infected with virus.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free [NBP2-80918]

Immunocytochemistry/ Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free [NBP2-80918]

Immunocytochemistry/Immunofluorescence: Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free [NBP2-80918] - PyMT-transgenic mouse mammary glands were stained in green for the PyMT protein. Staining showed good membrane/cytoplasmic localization with moderate signal for most cells in mammary tumor. Blocking performed with 20% goat serum. Image from verified customer review. Image from the standard format of this antibody.
Immunohistochemistry: Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free [NBP2-80918]

Immunohistochemistry: Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free [NBP2-80918]

Immunohistochemistry: Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free [NBP2-80918] - Darpp-32 and t-Darpp expression inPpp1r1bknockout mice. Mammary tumor samples were formalin-fixed and serial sections were stained for Darpp-32 and PyMT (5x magnification in a 6 x 6 tile, scale bar = 2000 um; images enlarged from the boxed regions are at

Applications for Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 19816927)

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Polyoma Virus, Medium T Antigen

Polyoma Viruses are a class of potentially oncogenic DNA viruses that often persist as latent infections in a host without causing disease, but may produce tumors in a host of a different species. Of particular interest, is that only the rodent Polyoma Viruses code for the production of PyMT (Medium T Antigen). This oncogenic protein of the Polyoma Virus is associated with cell membranes and interacts with a variety of cellular proteins involved in mitogenic signaling, such as tyrosine kinases c-Src, c-Yes, and Fyn. Medium T Antigen may therefore mimic the function of cellular tyrosine kinase growth factor receptors, like the platelet-derived growth factor or epidermal growth factor receptor (PMCID: PMC191984). Furthermore, it has been found that the Medium T Antigen may have a mechanism for inactivating p53, as it seems to be a characteristic of oncogenic DNA viruses (PMID: 22261839).

Alternate Names

Middle t antigen, MPyVgp2, Polyoma virus middle T antigen, Polyomavirus medium t antigen, Polyomavirus middle t antigen

Additional Polyoma Virus, Medium T Antigen Products

Product Documents for Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Polyoma Virus, Medium T Antigen Antibody (PyMT) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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