PRAS40 Antibody (2Y8Y3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16179

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16179-100ul
NBP3-16179-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2Y8Y3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PRAS40 Antibodies »

Product Summary for PRAS40 Antibody (2Y8Y3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-256 of human PRAS40 (Q96B36). STDDGSLSEETPAGPPTCSVPPASALPTQQYAKSLPVSVPVWGFKEKRTEARSSDEENGPPSSPDLDRIAASMRALVLREAEDTQVFGDLPRPRLNTSDFQKLKRKY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRAS40 Antibody (2Y8Y3)

Western Blot: PRAS40 Antibody (2Y8Y3) [NBP3-16179]

Western Blot: PRAS40 Antibody (2Y8Y3) [NBP3-16179]

Western Blot: PRAS40 Antibody (2Y8Y3) [NBP3-16179] - Western blot analysis of extracts of various cell lines, using AKT1S1/PRAS40 Rabbit mAb (NBP3-16179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for PRAS40 Antibody (2Y8Y3)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PRAS40

Proline Rich Akt Substrate (PRAS40) is a 40 kDa substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3 kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It may bind SH3 and WW domain containing proteins resulting in a change of function. Activated Akt phosphorylates PRAS40 on threonine 246. Mutation of PRAS40 threonine 246 has been shown to be apoptotic.

Long Name

40 kDa Proline-rich Akt1 Substrate

Alternate Names

AKT1S1, Lobe

Gene Symbol

AKT1S1

Additional PRAS40 Products

Product Documents for PRAS40 Antibody (2Y8Y3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRAS40 Antibody (2Y8Y3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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