PRAS40 [p Thr246] Antibody (JE53-94)

Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790]

Western Blot: PRAS40 [p Thr246] Antibody (JE53-94) [NBP3-32790] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32790) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: HeLa serum starved and treated with 100nM insulin for 5 minutes cell lysate
Lane 3: HeLa serum starved and treated with 100nM insulin for 5 minutes, then treated with pp for 1 hour cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 27 kDa
Observed band size: 42 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32790) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.