PRAS40 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19924

Novus Biologicals, part of Bio-Techne
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NBP2-19924
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (90%), Primate (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRAS40 Antibodies »

Product Summary for PRAS40 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PRAS40. The exact sequence is proprietary.

Reactivity Notes

Rat (87%).

Localization

Cytoplasm; cytosol

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRAS40 Antibody

Western Blot: PRAS40 Antibody [NBP2-19924]

Western Blot: PRAS40 Antibody [NBP2-19924]

Western Blot: PRAS40 Antibody [NBP2-19924] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-19924]

Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-19924]

Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-19924] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
Western Blot: PRAS40 Antibody [NBP2-19924]

Western Blot: PRAS40 Antibody [NBP2-19924]

Western Blot: PRAS40 Antibody [NBP2-19924] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PRAS40 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for PRAS40 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PRAS40

AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM]

Long Name

40 kDa Proline-rich Akt1 Substrate

Alternate Names

AKT1S1, Lobe

Gene Symbol

AKT1S1

Additional PRAS40 Products

Product Documents for PRAS40 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PRAS40 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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