Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PRAS40 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1. The immunogen is located within amino acids 190 - 240 of AKT1S1. Amino Acid Squence: RTEARSSDEENGPPSSPDL

Specificity

At least three isoforms of AKT1S1 are known to exist.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRAS40 Antibody - BSA Free

Western Blot: PRAS40 AntibodyBSA Free [NBP2-41221]

Western Blot: PRAS40 AntibodyBSA Free [NBP2-41221]

Western Blot: PRAS40 Antibody [NBP2-41221] - Western blot analysis of AKT1S1 in human brain tissue lysate with AKT1S1 antibody at (A) 1 and (B) 2 ug/mL.
Immunohistochemistry-Paraffin: PRAS40 Antibody - BSA Free [NBP2-41221]

Immunohistochemistry-Paraffin: PRAS40 Antibody - BSA Free [NBP2-41221]

Immunohistochemistry-Paraffin: PRAS40 Antibody [NBP2-41221] - Rat brain tissue with AKT1S1 antibody at 2.5 ug/ml.

Immunohistochemistry: PRAS40 Antibody [NBP2-41221] -

Immunohistochemistry: PRAS40 Antibody [NBP2-41221] - PRAS40 in rat brain tissue with PRAS 40 antibody at 20 ug/ml.
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Applications for PRAS40 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1 - 2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRAS40

Proline Rich Akt Substrate (PRAS40) is a 40 kDa substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3 kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It may bind SH3 and WW domain containing proteins resulting in a change of function. Activated Akt phosphorylates PRAS40 on threonine 246. Mutation of PRAS40 threonine 246 has been shown to be apoptotic.

Long Name

40 kDa Proline-rich Akt1 Substrate

Alternate Names

AKT1S1, Lobe

Gene Symbol

AKT1S1

Additional PRAS40 Products

Product Documents for PRAS40 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PRAS40 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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