PRAS40 [p Ser183] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13077

Novus Biologicals, part of Bio-Techne
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NBP3-13077
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PRAS40 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser183 of human PRAS40. The exact sequence is proprietary.

Modification

p Ser 183

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PRAS40 [p Ser183] Antibody

Western Blot: PRAS40 [p Ser183] Antibody [NBP3-13077]

Western Blot: PRAS40 [p Ser183] Antibody [NBP3-13077]

Western Blot: PRAS40 [p Ser183] Antibody [NBP3-13077] - Untreated (-) and treated (+) 293T whole cell extracts (40 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PRAS40 (phospho Ser183) antibody (NBP3-13077) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for PRAS40 [p Ser183] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRAS40

Proline Rich Akt Substrate (PRAS40) is a 40 kDa substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3 kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It may bind SH3 and WW domain containing proteins resulting in a change of function. Activated Akt phosphorylates PRAS40 on threonine 246. Mutation of PRAS40 threonine 246 has been shown to be apoptotic.

Long Name

40 kDa Proline-rich Akt1 Substrate

Alternate Names

AKT1S1, Lobe

Gene Symbol

AKT1S1

Additional PRAS40 Products

Product Documents for PRAS40 [p Ser183] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PRAS40 [p Ser183] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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