Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Chicken, Guinea Pig, Primate, Rabbit, Sheep

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # Alpha PR6

Format

BSA Free

Concentration

LYOPH mg/ml

View all Progesterone R/NR3C3 Antibodies »

Product Specifications

Immunogen

Progesterone receptor purified from chick oviduct cytosol.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 18980817). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

Detects the B form of the progesterone receptor (PR). This does not cross-react with estrogen receptor or glucocorticoid receptor.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Progesterone R/NR3C3 Antibody (Alpha PR6) - BSA Free

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (Alpha PR6) - BSA Free [NB120-2765]

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (Alpha PR6) - BSA Free [NB120-2765]

Immunohistochemistry-Paraffin: Progesterone R/NR3C3 Antibody (Alpha PR6) [NB120-2765] - Staining of human uterus tissue.

Applications for Progesterone R/NR3C3 Antibody (Alpha PR6) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

20 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Immunoprecipitation

1:10 - 1:500

Western Blot

1 ug/ml
Application Notes
WB: Detects an approx. 110 kDa protein representing the B form of the PR in chickens and rabbits as well a 120 kDa doublet or triplet bands in humans.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with sterilized PBS. reconstitute with 100ul. the 0.025mg size is provided already reconstituted at 1 mg/ml in PBS with 0.05% Sodium Azide.

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Progesterone R/NR3C3

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Long Name

Progesterone Receptor

Alternate Names

NR3C3, PGR, ProgesteroneR

Entrez Gene IDs

5241 (Human); 18667 (Mouse); 25154 (Rat)

Gene Symbol

PGR

UniProt

P06401

Additional Progesterone R/NR3C3 Products

Product Documents for Progesterone R/NR3C3 Antibody (Alpha PR6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Progesterone R/NR3C3 Antibody (Alpha PR6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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