Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73636
![Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636]](https://resources.bio-techne.com/images/products/Proteasome-19S-S3-Antibody-OTI2A2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73636-img0003.jpg "Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636]")
![Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636]](https://resources.bio-techne.com/images/products/Proteasome-19S-S3-Antibody-OTI2A2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73636-img0004.jpg "Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636]")
![Western Blot: Proteasome 19S S3 Antibody (OTI2A2)Azide and BSA Free [NBP2-73636]](https://resources.bio-techne.com/images/products/Proteasome-19S-S3-Antibody-OTI2A2-Azide-and-BSA-Free-Western-Blot-NBP2-73636-img0002.jpg "Western Blot: Proteasome 19S S3 Antibody (OTI2A2)Azide and BSA Free [NBP2-73636]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Canine, Monkey, Primate
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2A2
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PSMD3(NP_002800) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
60.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free
Western Blot: Proteasome 19S S3 Antibody (OTI2A2)Azide and BSA Free [NBP2-73636]
Western Blot: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S S3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Prot![Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636]](https://resources.bio-techne.com/images/products/Proteasome-19S-S3-Antibody-OTI2A2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73636-img0005.jpg "Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636]")
Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636]
Flow Cytometry: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Proteasome 19S S3 antibody, and then analyzed by flow cytometry.![Western Blot: Proteasome 19S S3 Antibody (OTI2A2)Azide and BSA Free [NBP2-73636]](https://resources.bio-techne.com/images/products/Proteasome-19S-S3-Antibody-OTI2A2-Azide-and-BSA-Free-Western-Blot-NBP2-73636-img0001.jpg "Western Blot: Proteasome 19S S3 Antibody (OTI2A2)Azide and BSA Free [NBP2-73636]")
Western Blot: Proteasome 19S S3 Antibody (OTI2A2)Azide and BSA Free [NBP2-73636]
Western Blot: Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free [NBP2-73636] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 19S S3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).Applications for Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Proteasome 19S S3
Alternate Names
26S proteasome regulatory subunit RPN3, 26S proteasome regulatory subunit S3, P58, proteasome (prosome, macropain) 26S subunit, non-ATPase, 3, Proteasome subunit p58, Rpn3, S3, 26S proteasome non-ATPase regulatory subunit 3, tissue specific transplantation antigen 2, TSTA2
Gene Symbol
PSMD3
Additional Proteasome 19S S3 Products
Product Documents for Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free
Product Specific Notices for Proteasome 19S S3 Antibody (OTI2A2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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