PSMB7 Antibody (OTI1E1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01832

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E1

Concentration

1 mg/ml

View all PSMB7 Antibodies »

Product Summary for PSMB7 Antibody (OTI1E1)

Immunogen

Human recombinant protein fragment corresponding to amino acids 58-277 of human PSMB7(NP_002790) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSMB7 Antibody (OTI1E1)

Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832]

Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832]

Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S beta 7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 20S beta 7.
Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832]

Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832]

Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Human prostate tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.
Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832]

Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832]

Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 20S beta 7 monoclonal antibody.
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Applications for PSMB7 Antibody (OTI1E1)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSMB7

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit). [provided by RefSeq].

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 7

Alternate Names

Macropain Chain Z, Proteasome Subunit Z

Entrez Gene IDs

5695 (Human)

Gene Symbol

PSMB7

Additional PSMB7 Products

Product Documents for PSMB7 Antibody (OTI1E1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PSMB7 Antibody (OTI1E1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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