PSMB7 Antibody (OTI1E1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73645

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PSMB7 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 58-277 of human PSMB7(NP_002790) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSMB7 Antibody (OTI1E1) - Azide and BSA Free

Western Blot: PSMB7 Antibody (OTI1E1)Azide and BSA Free [NBP2-73645]

Western Blot: PSMB7 Antibody (OTI1E1)Azide and BSA Free [NBP2-73645]

Western Blot: PSMB7 Antibody (OTI1E1) - Azide and BSA Free [NBP2-73645] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S beta 7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti
Immunohistochemistry: PSMB7 Antibody (OTI1E1) - Azide and BSA Free [NBP2-73645]

Immunohistochemistry: PSMB7 Antibody (OTI1E1) - Azide and BSA Free [NBP2-73645]

Immunohistochemistry: PSMB7 Antibody (OTI1E1) - Azide and BSA Free [NBP2-73645] - Staining of paraffin-embedded Human colon tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.
Western Blot: PSMB7 Antibody (OTI1E1)Azide and BSA Free [NBP2-73645]

Western Blot: PSMB7 Antibody (OTI1E1)Azide and BSA Free [NBP2-73645]

Western Blot: PSMB7 Antibody (OTI1E1) - Azide and BSA Free [NBP2-73645] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 20S beta 7 monoclonal antibody.
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Applications for PSMB7 Antibody (OTI1E1) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSMB7

PSMB7 (proteasome subunit, beta-type 7), also called proteasome subunit Z, is a widely expressed, non-catalytic component of the 20S proteasome core within the 26S proteasome that stabilizes late stages of proteasome assembly. Human PSMB7 is synthesized as a 277 amino acid (aa) protein with a 43 aa propeptide and a 234 active protein that contains one potential tyrosine phosphorylation site. Total PSMB7 expression is positively correlated with chemotherapy resistance in human breast cancer.

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 7

Alternate Names

Macropain Chain Z, Proteasome Subunit Z

Gene Symbol

PSMB7

Additional PSMB7 Products

Product Documents for PSMB7 Antibody (OTI1E1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PSMB7 Antibody (OTI1E1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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