PTEN Antibody (SJ19-03)

Immunohistochemistry: PTEN Antibody (SJ19-03) [NBP3-32872]

Immunohistochemistry: PTEN Antibody (SJ19-03) [NBP3-32872] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32872) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32872) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: PTEN Antibody (SJ19-03) [NBP3-32872]

Immunocytochemistry/ Immunofluorescence: PTEN Antibody (SJ19-03) [NBP3-32872] - ICC staining of PTEN in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32872, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Western Blot: PTEN Antibody (SJ19-03) [NBP3-32872]

Western Blot: PTEN Antibody (SJ19-03) [NBP3-32872] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32872) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MDA-MB-468 cell lysate (negative)
Lane 3: A431 cell lysate
Lane 4: HeLa cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lane 7: PC-12 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 47 kDa
Observed band size: 55 kDa

Exposure time: 1 minute 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.