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PTK7/CCK4 Antibody (OTI2E7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01486

Novus Biologicals, part of Bio-Techne
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NBP2-01486
Conjugate
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2E7

Concentration

1 mg/ml

Product Summary for PTK7/CCK4 Antibody (OTI2E7)

Immunogen

Full length human recombinant protein of human PTK7(NP_690619) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PTK7/CCK4 Antibody (OTI2E7)

Western Blot: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486]

Western Blot: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486]

Western Blot: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - Analysis of extracts (35ug) from 9 different cell lines by using anti-CCK4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486]

Immunocytochemistry/ Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486]

Immunocytochemistry/Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CCK4.
Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486]

Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486]

Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) [NBP2-01486] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CCK4 antibody, and then analyzed by flow cytometry.

Applications for PTK7/CCK4 Antibody (OTI2E7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:200-500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PTK7/CCK4

Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene is a member of this subgroup of tyrosine kinases and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene. [provided by RefSeq].

Long Name

Protein Tyrosine Kinase 7

Alternate Names

CCK4

Entrez Gene IDs

5754 (Human)

Gene Symbol

PTK7

Additional PTK7/CCK4 Products

Product Documents for PTK7/CCK4 Antibody (OTI2E7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PTK7/CCK4 Antibody (OTI2E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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