PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73708
![Western Blot: PTK7/CCK4 Antibody (OTI2E7)Azide and BSA Free [NBP2-73708]](https://resources.bio-techne.com/images/products/PTK7-CCK4-Antibody-OTI2E7-Azide-and-BSA-Free-Western-Blot-NBP2-73708-img0001.jpg "Western Blot: PTK7/CCK4 Antibody (OTI2E7)Azide and BSA Free [NBP2-73708]")
![Western Blot: PTK7/CCK4 Antibody (OTI2E7)Azide and BSA Free [NBP2-73708]](https://resources.bio-techne.com/images/products/PTK7-CCK4-Antibody-OTI2E7-Azide-and-BSA-Free-Western-Blot-NBP2-73708-img0002.jpg "Western Blot: PTK7/CCK4 Antibody (OTI2E7)Azide and BSA Free [NBP2-73708]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2E7
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary for PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free
Immunogen
Full length human recombinant protein of human PTK7(NP_690619) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free
Western Blot: PTK7/CCK4 Antibody (OTI2E7)Azide and BSA Free [NBP2-73708]
Western Blot: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CCK4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CCK4.![Immunocytochemistry/ Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708]](https://resources.bio-techne.com/images/products/PTK7-CCK4-Antibody-OTI2E7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73708-img0004.jpg "Immunocytochemistry/ Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708]")
Immunocytochemistry/ Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708]
Immunocytochemistry/Immunofluorescence: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CCK4.![Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708]](https://resources.bio-techne.com/images/products/PTK7-CCK4-Antibody-OTI2E7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73708-img0003.jpg "Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708]")
Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708]
Flow Cytometry: PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free [NBP2-73708] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CCK4 antibody, and then analyzed by flow cytometry.Applications for PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:200-500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PTK7/CCK4
Long Name
Protein Tyrosine Kinase 7
Alternate Names
CCK4
Gene Symbol
PTK7
Additional PTK7/CCK4 Products
Product Documents for PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free
Product Specific Notices for PTK7/CCK4 Antibody (OTI2E7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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