PTP1B/PTPN1 Antibody (OTI1D10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01056
![Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Flow-Cytometry-NBP2-01056-img0001.jpg "Flow Cytometry: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]")
![Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Western-Blot-NBP2-01056-img0009.jpg "Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Rat, Canine, Monkey, Primate
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI1D10
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human PTPN1(NP_002818) produced in HEK293 cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
49.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PTP1B/PTPN1 Antibody (OTI1D10)
Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]
Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and PTPN1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-PTPN1 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody as a loading control (1:500). Image using the Azide and BSA Free format of this antibody.![Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Immunocytochemistry-Immunofluorescence-NBP2-01056-img0006.jpg "Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]")
Immunocytochemistry/ Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]
Immunocytochemistry/Immunofluorescence: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PTPN1.![Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Immunohistochemistry-Paraffin-NBP2-01056-img0005.jpg "Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]")
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]
Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056] - Staining of paraffin-embedded Human lymphoma tissue using anti-PTPN1 mouse monoclonal antibody.![Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Western-Blot-NBP2-01056-img0002.jpg "Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]")
![Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Western-Blot-NBP2-01056-img0007.jpg "Western Blot: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]")
![Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Immunohistochemistry-Paraffin-NBP2-01056-img0003.jpg "Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]")
![Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]](https://resources.bio-techne.com/images/products/PTP1B-PTPN1-Antibody-OTI1D10-Immunohistochemistry-Paraffin-NBP2-01056-img0004.jpg "Immunohistochemistry-Paraffin: PTP1B/PTPN1 Antibody (OTI1D10) [NBP2-01056]")
Applications for PTP1B/PTPN1 Antibody (OTI1D10)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PTP1B
Long Name
Protein Tyrosine Phosphatase 1B
Alternate Names
PTPN1
Entrez Gene IDs
5770 (Human)
Gene Symbol
PTPN1
Additional PTP1B Products
Product Documents for PTP1B/PTPN1 Antibody (OTI1D10)
Product Specific Notices for PTP1B/PTPN1 Antibody (OTI1D10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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