PTP1B/PTPN1 Antibody (OTI1D10) [Janelia Fluor® 669]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73729JF669

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP2-01056

Alexa Fluor 350

NBP2-73729AF350

Alexa Fluor 405

NBP2-73729AF405

Alexa Fluor 488

NBP2-73729AF488

Alexa Fluor 532

NBP2-73729AF532

Alexa Fluor 594

NBP2-73729AF594

Alexa Fluor 647

NBP2-73729AF647

Alexa Fluor 700

NBP2-73729AF700

Alexa Fluor 750

NBP2-73729AF750

Allophycocyanin

NBP2-73729APC

Allophycocyanin/Cy7

NBP2-73729APCCY7

Biotin

NBP2-73729B

CoraFluor 1

NBP2-73729CL1

DyLight 350

NBP2-73729UV

DyLight 405

NBP2-73729V

DyLight 488

NBP2-73729G

DyLight 550

NBP2-73729R

DyLight 594

NBP2-73729DL594

DyLight 650

NBP2-73729C

DyLight 680

NBP2-73729FR

DyLight 755

NBP2-73729IR

FITC

NBP2-73729F

HRP

NBP2-73729H

Janelia Fluor 525

NBP2-73729JF525

Janelia Fluor 549

NBP2-73729JF549

Janelia Fluor 585

NBP2-73729JF585

Janelia Fluor 635

NBP2-73729JF635

Janelia Fluor 646

NBP2-73729JF646

mFluor Violet 450 SE

NBP2-73729MFV450

mFluor Violet 500 SE

NBP2-73729MFV500

mFluor Violet 610 SE

NBP2-73729MFV610

PE

NBP2-73729PE

PE/Atto594

NBP2-73729PEATT594

PE/Cy5.5

NBP2-73729PECY55

PE/Cy7

NBP2-73729PECY7

PerCP

NBP2-73729PCP

Forumulation

Catalog #

Azide and BSA Free

NBP2-73729

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Rat, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Janelia Fluor 669

Antibody Source

Monoclonal Mouse IgG_2A Clone # OTI1D10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PTP1B Antibodies »

Product Summary for PTP1B/PTPN1 Antibody (OTI1D10) [Janelia Fluor® 669]

Immunogen

Full length human recombinant protein of human PTPN1(NP_002818) produced in HEK293 cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Applications for PTP1B/PTPN1 Antibody (OTI1D10) [Janelia Fluor® 669]

Application

Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: PTP1B

The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Gene Symbol

PTPN1

Additional PTP1B Products

Product Documents for PTP1B/PTPN1 Antibody (OTI1D10) [Janelia Fluor® 669]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for PTP1B/PTPN1 Antibody (OTI1D10) [Janelia Fluor® 669]

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

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