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PYK2/FAK2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12969

Novus Biologicals, part of Bio-Techne
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NBP3-12969

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for PYK2/FAK2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PYK2/FAK2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for PYK2/FAK2 Antibody

Western Blot: PYK2/FAK2 Antibody [NBP3-12969]

Western Blot: PYK2/FAK2 Antibody [NBP3-12969]

Western Blot: PYK2/FAK2 Antibody [NBP3-12969] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2/FAK2 antibody (NBP3-12969) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: PYK2/FAK2 Antibody [NBP3-12969]

Immunocytochemistry/ Immunofluorescence: PYK2/FAK2 Antibody [NBP3-12969]

Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PYK2/FAK2 stained by PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP3-12969]

Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP3-12969]

Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody [NBP3-12969] - PYK2/FAK2 antibody detects PYK2/FAK2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. PYK2/FAK2 antibody (NBP3-12969) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for PYK2/FAK2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PYK2/FAK2

Proline rich protein tyrosine kinase (Pyk2) is a focal adhesion kinase (FAK2) related protein kinase protein also known as cell adhesion kinase (CAK ), related adhesion focal tyrosine kinase (RAFTK), or calcium-dependent PTK (CADTK) (3.4). Highly expre ed in the nervous system, protein tyrosine kinase (Pyk2) is phosphorylated on the tyrosine residue by a variety of G-protein-coupled receptors & extracellular signals that increase the level of Ca+ intracellulary (1,2)

Long Name

Proline-rich Tyrosine Kinase 2

Alternate Names

CADTK, CAKB, FADK2, FAK2, FRNK, PTK2B, RAFTK

Gene Symbol

PTK2B

Additional PYK2/FAK2 Products

Product Documents for PYK2/FAK2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PYK2/FAK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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