Rad21 Antibody (CM110-2C10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-386
![Western Blot: Rad21 Antibody (CM110-2C10)BSA Free [NB100-386]](https://resources.bio-techne.com/images/products/Rad21-Antibody-CM110-2C10-Western-Blot-NB100-386-img0003.jpg "Western Blot: Rad21 Antibody (CM110-2C10)BSA Free [NB100-386]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # CM110-2C10
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
An N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21).
Localization
Nuclear and cytoplasm
Specificity
NB100-386 is specific for both the nuclear hRad21 protein and the cleaved cytoplasmic hRad21 protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Rad21 Antibody (CM110-2C10) - BSA Free
Western Blot: Rad21 Antibody (CM110-2C10)BSA Free [NB100-386]
Western Blot: Rad21 Antibody (CM110-2C10) [NB100-386] - Western blot with monoclonal anti-Rad21 (NB100-386) in HEK293 lysates.![Simple Western: Rad21 Antibody (CM110-2C10)BSA Free [NB100-386]](https://resources.bio-techne.com/images/products/Rad21-Antibody-CM110-2C10-Simple-Western-NB100-386-img0004.jpg "Simple Western: Rad21 Antibody (CM110-2C10)BSA Free [NB100-386]")
Simple Western: Rad21 Antibody (CM110-2C10)BSA Free [NB100-386]
Simple Western: Rad21 Antibody (CM110-2C10) [NB100-386] - Simple Western lane view shows a specific band for Rad21 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for Rad21 Antibody (CM110-2C10) - BSA Free
Application
Recommended Usage
Simple Western
1:50
Western Blot
1:500 (ECL)
Application Notes
This Rad21 (CM110-2C10) antibody can be used in Western blot, where two bands are seen. The full-length Rad21 is seen around 130 kDa, while the cleaved Rad21 is seen around 70 kDa.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Rad21
Alternate Names
double-strand-break repair protein rad21 homolog, hHR21FLJ25655, HR21, KIAA0078RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, NXP-1, NXP1HRAD21, protein involved in DNA double-strand break repair, RAD21 homolog (S. pombe), SCC1 homolog, SCC1FLJ40596
Gene Symbol
RAD21
UniProt
Additional Rad21 Products
Product Documents for Rad21 Antibody (CM110-2C10) - BSA Free
Product Specific Notices for Rad21 Antibody (CM110-2C10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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