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Rad21 Antibody (CM110-2C10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80922

Novus Biologicals, part of Bio-Techne
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NBP2-80922

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CM110-2C10

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for Rad21 Antibody (CM110-2C10) - Azide and BSA Free

Immunogen

An N-terminal fusion protein of the C-terminal fragment of Scc1 subunit human cohesin (amino acids 544-631 of hRad21).

Localization

Nuclear and cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Rad21 Antibody (CM110-2C10) - Azide and BSA Free

Western Blot: Rad21 Antibody (CM110-2C10)Azide and BSA Free [NBP2-80922]

Western Blot: Rad21 Antibody (CM110-2C10)Azide and BSA Free [NBP2-80922]

Western Blot: Rad21 Antibody (CM110-2C10) - Azide and BSA Free [NBP2-80922] - Western blot with monoclonal anti-Rad21 (NB100-386) in HEK293 lysates. Image from the standard format of this antibody.
Simple Western: Rad21 Antibody (CM110-2C10)Azide and BSA Free [NBP2-80922]

Simple Western: Rad21 Antibody (CM110-2C10)Azide and BSA Free [NBP2-80922]

Simple Western: Rad21 Antibody (CM110-2C10) - Azide and BSA Free [NBP2-80922] - Simple Western lane view shows a specific band for Rad21 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

Applications for Rad21 Antibody (CM110-2C10) - Azide and BSA Free

Application
Recommended Usage

Simple Western

1:50

Western Blot

1:500 (ECL)
Application Notes
This Rad21 (CM110-2C10) antibody can be used in Western blot, where two bands are seen. The full-length Rad21 is seen around 130 kDa, while the cleaved Rad21 is seen around 70 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rad21

The cohesin complex plays an important role in tethering sister chromatids during the prophase through anaphase stages of mitosis, making certain that genomic information is replicated accurately. As the cellular division process continues, separase destroys the cohesin complex by means of cleavaging its Scc1 subunit (Mcd1/Scc1/hRad21), allowing the chromatids to separate and divide with the cell. During apoptosis the C-terminal portion of hRad21 is cleaved and translocated to the cytoplasm. This cleavage and translocation may act as a nuclear signal that promotes and accelerates subsequent events of apoptosis (2).

Alternate Names

double-strand-break repair protein rad21 homolog, hHR21FLJ25655, HR21, KIAA0078RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, NXP-1, NXP1HRAD21, protein involved in DNA double-strand break repair, RAD21 homolog (S. pombe), SCC1 homolog, SCC1FLJ40596

Gene Symbol

RAD21

Additional Rad21 Products

Product Documents for Rad21 Antibody (CM110-2C10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rad21 Antibody (CM110-2C10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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