RANK/TNFRSF11A Antibody (9A725) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56029

Novus Biologicals, part of Bio-Techne
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NB100-56029
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Avian

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow (Cell Surface), Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow (Cell Surface), Flow Cytometry Control, Immunohistochemistry-Paraffin, Western Blot

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 9A725

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RANK/TNFRSF11A Antibodies »

Product Specifications

Immunogen

A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24376119). Not yet tested in other species.

Specificity

In RAW cells, a 97 kDa is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RANK/TNFRSF11A Antibody (9A725) [PE]

Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [PE] [NB100-56029] - An intracellular stain was performed on K-562 cells with RANK/TNFRSF11A (9A725) antibody NB100-56029 (blue) and a matched isotype control NBP1-97005PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [PE] [NB100-56029] - Intracellular flow analysis of RANK in RAW cells using 0.5 ug of RANK antibody. Green histogram represents isotype control antibody (NB100-56029); red represents RANK antibody.

Applications for RANK/TNFRSF11A Antibody (9A725) [PE]

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Immunohistochemistry

1:10-1:500
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: RANK/TNFRSF11A

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2.

Long Name

Receptor Activator of NF-kappa-B

Alternate Names

CD265, ODFR, TNFRSF11A, TRANCE R

Gene Symbol

TNFRSF11A

Additional RANK/TNFRSF11A Products

Product Documents for RANK/TNFRSF11A Antibody (9A725) [PE]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RANK/TNFRSF11A Antibody (9A725) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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