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RANK/TNFRSF11A Antibody (9A725) [Alexa Fluor® 647]

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56508AF647

Novus Biologicals, part of Bio-Techne
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NB100-56508AF647

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Avian

Applications

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 9A725

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for RANK/TNFRSF11A Antibody (9A725) [Alexa Fluor® 647]

Immunogen

A fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK (NP_003830).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 24376119). Avian reactivity reported in scientific literature (PMID: 31214852).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RANK/TNFRSF11A Antibody (9A725) [Alexa Fluor® 647]

Flow Cytometry: RANK/TNFRSF11A Antibody (9A725) [Alexa Fluor® 647] [NB100-56508AF647] - An intracellular stain was performed on Raw264.7 cells with RANK/TNFRSF11A [9A725] Antibody NB100-56508AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
RANK/TNFRSF11A Antibody (9A725) [Alexa Fluor® 647]

RANK/TNFRSF11A (9A725) in Raw 264.7 Mouse Cell Line.

RANK/TNFRSF11A (9A725) was detected in immersion fixed Raw 264.7 mouse macrophage cell line using Mouse anti-RANK/TNFRSF11A (9A725) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NB100-56508AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for RANK/TNFRSF11A Antibody (9A725) [Alexa Fluor® 647]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Cell Surface)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: RANK/TNFRSF11A

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Receptor activator of NF-kB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family (1). RANK ligand (RANKL/TRANCE/ OPGL) (mAbs to RANKL is also available from Imgenex, Cat # IMG-133) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6 (3,4). Overexpression of RANK activates NF-kB and c-Jun-terminal kinase (JNK) pathways (3). Recent studies have shown that RANK interaction with TRAF6 activates NF-kB, whereas JNK activation is mediated through binding of RANK to TRAF2 (4).

Long Name

Receptor Activator of NF-kappa-B

Alternate Names

CD265, ODFR, TNFRSF11A, TRANCE R

Gene Symbol

TNFRSF11A

Additional RANK/TNFRSF11A Products

Product Documents for RANK/TNFRSF11A Antibody (9A725) [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RANK/TNFRSF11A Antibody (9A725) [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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