Ras Antibody (6J9I4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15909

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15909-100ul
NBP3-15909-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6J9I4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ras Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-188 of human NRAS (P01111). MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLNSSDDGTQGCMGLPCVV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ras Antibody (6J9I4)

Western Blot: Ras Antibody (6J9I4) [NBP3-15909]

Western Blot: Ras Antibody (6J9I4) [NBP3-15909]

Western Blot: Ras Antibody (3G8L8) [NBP3-15909] - Western blot analysis of extracts of various cell lines, using Ras Rabbit mAb (NBP3-15909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunocytochemistry/ Immunofluorescence: Ras Antibody (6J9I4) [NBP3-15909]

Immunocytochemistry/ Immunofluorescence: Ras Antibody (6J9I4) [NBP3-15909]

Immunocytochemistry/Immunofluorescence: Ras Antibody (3G8L8) [NBP3-15909] - Immunofluorescence analysis of NIH-3T3 cells using Ras Rabbit mAb (NBP3-15909) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: Ras Antibody (6J9I4) [NBP3-15909]

Western Blot: Ras Antibody (6J9I4) [NBP3-15909]

Western Blot: Ras Antibody (3G8L8) [NBP3-15909] - Western blot analysis of extracts of various cell lines, using Ras Rabbit mAb (NBP3-15909) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Applications for Ras Antibody (6J9I4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ras

The Ras proteins (H-Ras, N-Ras, and two splice variants of K-Ras) define a closely related family of 21 kDa GTPases that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. Receptor tyrosine kinases and G-protein coupled receptors activate Ras, and effectors include Raf and PI 3-kinase. Activating mutations of Ras, which impair GTPase activity and stabilize the active state, are found in nearly one-third of all human cancers.

Long Name

v-Ki-ras2 Kirsten Rat Sarcoma Viral Oncogene Jomolog

Alternate Names

c-K-ras, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, Ki-Ras, KRAS, KRAS1, KRAS2, Oncogene Ras, RASK2

Gene Symbol

KRAS

Additional Ras Products

Product Documents for Ras Antibody (6J9I4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Ras Antibody (6J9I4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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