Ras Antibody - G12D Mutant

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13102

Novus Biologicals, part of Bio-Techne
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NBP3-13102
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Ras Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding mutant G12D of human K-Ras. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Ras Antibody - G12D Mutant

Western Blot: Ras AntibodyG12D Mutant [NBP3-13102]

Western Blot: Ras AntibodyG12D Mutant [NBP3-13102]

Western Blot: Ras Antibody - G12D Mutant [NBP3-13102] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras (G12D Mutant Specific) antibody (NBP3-13102) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Ras Antibody - G12D Mutant

Western Blot: Ras Antibody - G12D Mutant [NBP3-13102] -

Western Blot: Ras Antibody - G12D Mutant [NBP3-13102] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ras antibody (NBP3-13102) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Ras Antibody - G12D Mutant

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ras

The Ras proteins (H-Ras, N-Ras, and two splice variants of K-Ras) define a closely related family of 21 kDa GTPases that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. Receptor tyrosine kinases and G-protein coupled receptors activate Ras, and effectors include Raf and PI 3-kinase. Activating mutations of Ras, which impair GTPase activity and stabilize the active state, are found in nearly one-third of all human cancers.

Long Name

v-Ki-ras2 Kirsten Rat Sarcoma Viral Oncogene Jomolog

Alternate Names

c-K-ras, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, Ki-Ras, KRAS, KRAS1, KRAS2, Oncogene Ras, RASK2

Additional Ras Products

Product Documents for Ras Antibody - G12D Mutant

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ras Antibody - G12D Mutant

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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