Rat splenocytes were stained with Anti-Rat CD3 APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat CD161 Biotinylated Monoclonal Antibody (Catalog # FAB9766B) or (B) Mouse IgG1 Biotinylated Isotype control (Catalog # IC002B)followed by Phycoerythrin-conjugated Streptavidin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat CD161 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Rat splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD161

CD161, also known as KLRB1, NK1.1, and NKR-P1A is a 40 kDa type II transmembrane glycoprotein that contains one C-type lectin domain in its extracellular region. CD161 is expressed as a disulfide-linked dimer on the surface of Th17 cells and NK cells as well as on subsets of CD1-restricted T cells, intestinal NT cells, peripheral memory T cells, monocytes, and dendritic cells. It binds to OCIL/CLEC2d, leading to an inhibition of NK cell-mediated cytotoxicity and IFN-gamma secretion. Alternatively, CD161 can enhance TCR activation to CD1d ligation. CD161+ cell populations are depleted in ulcerative colitis, Grave's disease, and AIDS, although CD161+ T cells are activated during asthmatic attacks. Additional related proteins are expressed in mouse but not human: the inhibitory NKR-P1B and D, and the stimulatory NKR-P1A, C, and F.

Alternate Names

CD161, CLEC5B, KLRB1, Ly59, NKR, NKRP1, NKRP1A

Entrez Gene IDs

3820 (Human); 25192 (Rat)

Gene Symbol

KLRB1

Additional CD161 Products

Product Documents for Rat CD161 Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat CD161 Biotinylated Antibody

For research use only

Citations
Reviews

Rat CD161 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB9766B

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD161 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat CD161 Biotinylated Antibody

Detection of Rat CD161 in Rat splenocytes by Flow Cytometry.