Rat splenocytes were stained with Anti-Rat CD3 APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat CD161 Monoclonal Antibody (Catalog # MAB9766) or (B) Mouse IgG1 Isotype control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat CD161 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Rat splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD161

CD161, also known as KLRB1, NK1.1, and NKR-P1A is a 40 kDa type II transmembrane glycoprotein that contains one C-type lectin domain in its extracellular region. CD161 is expressed as a disulfide-linked dimer on the surface of Th17 cells and NK cells as well as on subsets of CD1-restricted T cells, intestinal NT cells, peripheral memory T cells, monocytes, and dendritic cells. It binds to OCIL/CLEC2d, leading to an inhibition of NK cell-mediated cytotoxicity and IFN-gamma secretion. Alternatively, CD161 can enhance TCR activation to CD1d ligation. CD161+ cell populations are depleted in ulcerative colitis, Grave's disease, and AIDS, although CD161+ T cells are activated during asthmatic attacks. Additional related proteins are expressed in mouse but not human: the inhibitory NKR-P1B and D, and the stimulatory NKR-P1A, C, and F.

Alternate Names

CD161, CLEC5B, KLRB1, Ly59, NKR, NKRP1, NKRP1A

Entrez Gene IDs

3820 (Human); 25192 (Rat)

Gene Symbol

KLRB1

Additional CD161 Products

Product Documents for Rat CD161 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Rat CD161 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Rat CD161 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9766

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD161 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat CD161 Antibody

Detection of Rat CD161 in Rat splenocytes by Flow Cytometry.