Rat ICAM-1/CD54 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB583
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 141011
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat ICAM-1/CD54
Met1-Thr493
Accession # Q00238
Met1-Thr493
Accession # Q00238
Specificity
Detects rat ICAM-1/CD54 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) ICAM‑1, rmICAM-2, recombinant human (rh) ICAM-2, rhICAM-3, rmVCAM-1, or rhPCAM-1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Rat ICAM-1/CD54 Antibody
Detection of Rat ICAM‑1/CD54 by Western Blot.
Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat ICAM-1/CD54 Monoclonal Antibody (Catalog # MAB583) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ICAM-1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Rat ICAM-1/CD54 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Rat liver tissue
Sample: Rat liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54
Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.
Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.
References
- Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
- Siu, G. et al. (1989) J. Immunol. 143:3813.
- Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Long Name
Intercellular Adhesion Molecule 1
Alternate Names
CD54, ICAM1
Gene Symbol
ICAM1
UniProt
Additional ICAM-1/CD54 Products
Product Documents for Rat ICAM-1/CD54 Antibody
Product Specific Notices for Rat ICAM-1/CD54 Antibody
For research use only
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