Rat IL-7R alpha/CD127 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8484
Conjugate
Catalog #
Key Product Details
Species Reactivity
Rat
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 717519
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha/CD127
Glu21-Asp239
Accession # NP_001099888
Glu21-Asp239
Accession # NP_001099888
Specificity
Detects rat IL‑7 R alpha/CD127in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Rat IL-7R alpha/CD127 Antibody
Detection of IL‑7 R alpha /CD127 in CD4+Rat Splenocytes by Flow Cytometry.
CD4+rat splenocytes were stained with Mouse Anti-Rat IL-7 R alpha /CD127 Monoclonal Antibody (Catalog # MAB8484, filled histogram) or Mouse IgG2A Isotype Control (Catalog # MAB003, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.Applications for Rat IL-7R alpha/CD127 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: CD4+ Rat Splenocytes
Sample: CD4+ Rat Splenocytes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-7R alpha/CD127
IL-7 R alpha (IL-7 receptor alpha; also CD127) is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gammac chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 aa type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III fibronectin domain (aa 129‑222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21‑239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.
Long Name
Interleukin 7 Receptor alpha
Alternate Names
CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha
Gene Symbol
IL7R
UniProt
Additional IL-7R alpha/CD127 Products
Product Documents for Rat IL-7R alpha/CD127 Antibody
Product Specific Notices for Rat IL-7R alpha/CD127 Antibody
For research use only
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