Rat splenocytes were stained with Anti-Rat CD4 APC-conjugated Antibody and either (A) Mouse Anti-Rat IL-7 Ra/CD127 PE-conjugated Monoclonal Antibody (Catalog # FAB8484P) or (B) Mouse IgG_2APhycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat IL-7R alpha/CD127 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Rat splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-7R alpha/CD127

IL-7 R alpha (IL-7 Receptor alpha), also known as CD127, is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8⁺ T cells, DN thymocytes, pre-B cells, Th1 CD4⁺ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gammac chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP Receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4⁺ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 amino acid (aa) type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III Fibronectin domain (aa 129-222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21-239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.

Long Name

Interleukin 7 Receptor alpha

Alternate Names

CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha

Entrez Gene IDs

3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)

Gene Symbol

IL7R

UniProt

NP_001099888 (Rat)

Additional IL-7R alpha/CD127 Products

Product Documents for Rat IL-7R alpha/CD127 PE-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Rat IL-7R alpha/CD127 PE-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Rat IL-7R alpha/CD127 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB8484P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all IL-7R alpha/CD127 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat IL-7R alpha/CD127 PE-conjugated Antibody

Detection of IL-7 R alpha/CD127 in Rat Splenocytes by Flow Cytometry.