Rat Neurexin 1 beta/NXRN1b Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4524
Key Product Details
Species Reactivity
Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neurexin 1 beta/NXRN1b
Ala47-Thr392
Accession # Q63373
Ala47-Thr392
Accession # Q63373
Specificity
Detects rat Neurexin 1 beta/NXRN1b in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant rat Neurexin 1 alpha is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Rat Neurexin 1 beta/NXRN1b Antibody
Detection of Rat Neurexin 1 beta/NXRN1b by Western Blot.
Western blot shows lysates of rat spinal cord tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat Neurexin 1 beta/NXRN1b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4524) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurexin 1 beta/NXRN1b at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Rat Neurexin 1 beta/NXRN1b Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Rat spinal cord tissue
Sample: Rat spinal cord tissue
Reviewed Applications
Read 1 review rated 4 using AF4524 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neurexin 1 beta/NXRN1b
Neurexin 1 beta is an 80 kDa member of the Neurexin family of proteins. It is expressed on neuronal presynaptic membranes and contributes to synapse formation through binding to postsynaptic neuroligins. An alternate start site in the Neurexin 1 gene generate an alpha- (long) and beta- (short) isoform. Mature rat Neurexin 1 beta is a type I transmembrane glycoprotein that is 468 amino acids (aa) in length. It has a 346 aa extracellular region (aa 47‑392) that contains one laminin-like G domain (aa 91‑264). Within the G domain, the presence or absence of a splice site (aa 201‑230) materially impacts neuroligin binding. Over aa 47‑392, rat Neurexin 1 beta is 100% and 98% aa identical to mouse and human Neurexin 1 beta, respectively.
Alternate Names
NXR1B, NXRN1b
Gene Symbol
NRXN1
UniProt
Additional Neurexin 1 beta/NXRN1b Products
Product Documents for Rat Neurexin 1 beta/NXRN1b Antibody
Product Specific Notices for Rat Neurexin 1 beta/NXRN1b Antibody
For research use only
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