Rat TIMP-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF580
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat TIMP-1
Cys24-Ala217
Accession # P30120
Cys24-Ala217
Accession # P30120
Specificity
Detects rat TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human (rh) TIMP‑1 and recombinant mouse TIMP-1 is observed and less than 1% cross-reactivity with rhTIMP-2, rhTIMP-3, and rhTIMP-4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat TIMP-1 Antibody
Detection of TIMP-1 in Rat Brain.
TIMP-1 was detected in perfusion fixed paraffin-embedded sections of Rat Brain using Goat Anti-Rat TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF580) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Rat TIMP-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of Rat Brain.
Sample: Perfusion fixed paraffin-embedded sections of Rat Brain.
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Rat TIMP-1 (Catalog # 580-RT), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Rat TIMP-1 (Catalog # 580-RT), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Rat TIMP-1 (Catalog # 580-RT)
Sample: Recombinant Rat TIMP-1 (Catalog # 580-RT)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-1
Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family: TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 32‑34 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.
Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP1
Gene Symbol
TIMP1
UniProt
Additional TIMP-1 Products
Product Documents for Rat TIMP-1 Antibody
Product Specific Notices for Rat TIMP-1 Antibody
For research use only
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