S-arrestin Antibody (PDS-1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2385

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PDS-1

Format

BSA Free

Concentration

1 mg/ml

View all S-arrestin Antibodies »

Product Summary for S-arrestin Antibody (PDS-1) - BSA Free

Immunogen

Porcine retinal S-arrestin [Swiss-Prot# P79260]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for S-arrestin Antibody (PDS-1) - BSA Free

Western Blot: S-arrestin Antibody (PDS-1)BSA Free [NB100-2385]

Western Blot: S-arrestin Antibody (PDS-1)BSA Free [NB100-2385]

Western Blot: S-arrestin Antibody (PDS-1) [NB100-2385] - Western blot analysis of Visual Arrestin expression in human retina tissue extract.
Immunohistochemistry-Paraffin: S-arrestin Antibody (PDS-1) - BSA Free [NB100-2385]

Immunohistochemistry-Paraffin: S-arrestin Antibody (PDS-1) - BSA Free [NB100-2385]

Immunohistochemistry-Paraffin: S-arrestin Antibody (PDS-1) [NB100-2385] - S-arrestin was detected in immersion fixed paraffin-embedded sections of human prostate cancer using Mouse Anti-Human S-arrestin (PDS-1) Monoclonal Antibody (Catalog # NB100-2385) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm.

Applications for S-arrestin Antibody (PDS-1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:100-1:200

Immunohistochemistry-Frozen

1:100-1:200

Western Blot

1:500-1:2000
Application Notes
This S-arrestin antibody is useful for the immunopurification of arrestin (PMID 3549614).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: S-arrestin

Arrestins family contains four types of soluble cytoplasmic proteins that act as negative regulators of GPCR signaling, wherein, cognate ligand binding stimulates GPCR phosphorylation, followed by arrestin binding to phospho-GPCR leading to receptor internalization and ultimately, the desensitization of GPCR signaling. Among four different types of mammalian arrestins, the visual proteins namely arrestin 1 (S-arrestin, S-antigen or SAG) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively, whereas the non-visual proteins arrestin 2 (beta-arrestin 1) and arrestin 3 (beta-arrestin 2) are ubiquitously expressed. S-arrestin is rod specific and constitutes major proteins of retinal rod outer segments and associate with photoactivated/phosphorylated rhodopsin, thereby preventing transducin-mediated phosphodiesterase activation. Besides retinal rod photoreceptor cell outer segment, S-arrestin is also expressed in the pineal gland where its function is largely unknown. Arrestin knockout mice develop photoreceptor cell degeneration because of continuous phototransduction upregulation, as a consequence of defective rhodopsin/opsin shut-off mechanism, and in human subjects, S-arrestin mutations have been linked to Oguchi disease and retinitis pigmentosa.

Alternate Names

arrestin 1, RP47,48 kDa protein, S-antigen; retina and pineal gland (arrestin), S-arrestin, visual arrestin

Gene Symbol

SAG

Additional S-arrestin Products

Product Documents for S-arrestin Antibody (PDS-1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for S-arrestin Antibody (PDS-1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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