Sarcalumenin Antibody (XIIC4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB120-2874

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse, Canine, Guinea Pig, Rabbit

Applications

Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # XIIC4

Format

BSA Free

Concentration

1 mg/ml

View all Sarcalumenin Antibodies »

Product Specifications

Immunogen

Purified rabbit sarcoplasmic reticulum membrane vesicles.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This specifically detects sarcalumenin and the 53 kDa glycoprotein splice variant.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Sarcalumenin Antibody (XIIC4) - BSA Free

Western Blot: Sarcalumenin Antibody (XIIC4)BSA Free [NB120-2874]

Western Blot: Sarcalumenin Antibody (XIIC4)BSA Free [NB120-2874]

Western Blot: Sarcalumenin Antibody (XIIC4) [NB120-2874] - WB analysis was performed on tissue extracts (30 ug lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Skeletal Muscle (Lane 3), Rat Skeletal Muscle (Lane 4), Mouse Kidney (Lane 5), Rat Kidney (Lane 6), Rat Lung (Lane 7), Rat Pancreas (Lane 8), Rat Stomach (Lane 9) and Rat Adipose (Lane 10). The blot was probed with this antibody.
Western Blot: Sarcalumenin Antibody (XIIC4)BSA Free [NB120-2874]

Western Blot: Sarcalumenin Antibody (XIIC4)BSA Free [NB120-2874]

Western Blot: Sarcalumenin Antibody (XIIC4) [NB120-2874] - WB analysis was performed on tissue extract (30 ug lysate) of Mouse Heart and was probed with this antibody.

Applications for Sarcalumenin Antibody (XIIC4) - BSA Free

Application
Recommended Usage

Immunofluorescence

1:500

Western Blot

1:500
Application Notes
WB: Detects an approx. 53 kDa and a 160 kDa protein representing the glycoprotein and sarcalumenin respectively, in rabbit skeletal muscle extracts. IF: Staining of sarcalumenin and its splice variant in rabbit skeletal muscle results in staining that co-localizes with calcium ATPase in the longitudinal sarcoplasmic reticulum (SR) and the terminal cisternae.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sarcalumenin

Sarcalumenin is a 160 kDa acidic calcium binding glycoprotein found to reside at the inner membrane of the sarcoplasmic reticulum in a calcium dependent manner.The gene encoding sarcalumenin also encodes an alternate splice variant, a 53 kDa glycoprotein which co-localizes with sarcalumenin. These two proteins are found in the longitudinal sarcoplasmic reticulum and the nonjunctional membranes of the terminal cisternae. They have been observed to co-localize with calcium-ATPase suggesting that they are involved in calcium transport and sequestration.

Alternate Names

sarcalumenin

Entrez Gene IDs

106393 (Mouse)

Gene Symbol

SRL

Additional Sarcalumenin Products

Product Documents for Sarcalumenin Antibody (XIIC4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sarcalumenin Antibody (XIIC4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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