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Sarcalumenin Antibody (XIIC4) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NB120-2874CL1

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse, Canine, Guinea Pig, Rabbit

Applications

Immunofluorescence, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG1 Clone # XIIC4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Purified rabbit sarcoplasmic reticulum membrane vesicles.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This specifically detects sarcalumenin and the 53 kDa glycoprotein splice variant.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Sarcalumenin Antibody (XIIC4) [CoraFluor™ 1]

Application
Recommended Usage

Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Sarcalumenin

Sarcalumenin is a 160 kDa acidic calcium binding glycoprotein found to reside at the inner membrane of the sarcoplasmic reticulum in a calcium dependent manner.The gene encoding sarcalumenin also encodes an alternate splice variant, a 53 kDa glycoprotein which co-localizes with sarcalumenin. These two proteins are found in the longitudinal sarcoplasmic reticulum and the nonjunctional membranes of the terminal cisternae. They have been observed to co-localize with calcium-ATPase suggesting that they are involved in calcium transport and sequestration.

Alternate Names

sarcalumenin

Gene Symbol

SRL

Additional Sarcalumenin Products

Product Documents for Sarcalumenin Antibody (XIIC4) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sarcalumenin Antibody (XIIC4) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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