SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12854

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 7A4D12

Format

BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Alpha variant (B.1.1.7) Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 Alpha Variant (B.1.1.7, UK) Spike protein.

Specificity

May cross-react with several virus of interest (VOI) variant lineages that contains P681H mutation, including B.11.318, B.1.621, B.1.621.1, P.3. But all of these lineages are rarely present in current pandemic.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images

ELISA: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK - BSA Free [NBP3-12854]

ELISA: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK - BSA Free [NBP3-12854]

ELISA: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK [NBP3-12854] - ELISA Validation of Alpha Variant Spike Antibodies with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and omicron variant (B.1.1.529), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, NBP3-12854, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 alpha variant spike antibody (NBP3-12854) can specifically detect alpha variant spike S1 protein, but not spike S1 protein of WT and other tested variants by ELISA.
Western Blot: SARS-CoV-2 Spike Antibody (7A4D12)Alpha Variant, B.1.1.7, UKBSA Free [NBP3-12854]

Western Blot: SARS-CoV-2 Spike Antibody (7A4D12)Alpha Variant, B.1.1.7, UKBSA Free [NBP3-12854]

Western Blot: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK [NBP3-12854] - WB Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 P681H Mutant Specific Spike antibody, NBP3-12854, 1 ug/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (NBP3-12854) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by WB.
ELISA: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK - BSA Free [NBP3-12854]

ELISA: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK - BSA Free [NBP3-12854]

ELISA: SARS-CoV-2 Spike Antibody (7A4D12) - Alpha Variant, B.1.1.7, UK [NBP3-12854] - ELISA Validation of P681H Mutant Specific Spike Antibodies with SARS-CoV-2 Alpha Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT and alpha variant (B.1.1.7, UK), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Alpha Variant Spike antibody, NBP3-12854, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5,000, incubate at RT for 1 hr. SARS-CoV-2 P681H Mutant Specific Spike antibody (NBP3-12854) can specifically detect alpha variant spike S1 protein, but not WT spike S1 protein by ELISA.
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Applications

Application
Recommended Usage

Western Blot

1 ug/mL
Application Notes
Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 (COVID-19) P681H Mutant Specific Spike antibody can specifically detect SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA and WB. It can also detect mutant peptide (681H), but not WT peptide (681P). All other applications and species not yet tested.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spike

The SARS-CoV-2 Spike protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1,2). The spike protein is the largest of the structural proteins, which also include the membrane (M), envelope (E), and nucleocapsid (N) proteins (1,2). The SARS-CoV-2 spike protein is a 1273 amino acid (aa) heterotrimeric class I fusion protein with each monomer having a theoretical molecular weight of approximately 180 kDa (1). The club-shaped spike protein contains several functional regions and domains including the S1 globular head region which contains the N-terminal receptor-binding domain (RBD) and the S2 stem region that contains the C-terminal fusion domain, two heptad regions, a transmembrane domain, and a cytoplasmic tail (1,2). The viral spike protein is critical for attachment of the virus with the host cell, resulting in fusion and virus entry into the cell (1,2). More specifically, the RBD of the spike protein is responsible for binding to the cell surface receptor angiotensin converting enzyme 2 (ACE2) (1,2). This spike-ACE2 interaction results in a conformational change permitting furin cleavage between the S1 and S2 domains and then cleavage at S2' by TMPRRS2, or another protease, allowing membrane fusion (1,2).

Given the critical role of the spike protein RBD in the interaction with the ACE2 receptor and viral entry, a number of neutralizing antibodies against the RBD have been developed as potential therapeutics for treating COVID-19 (3). These antibodies bind the RBD domain on the S1 subunit inhibiting the interaction with ACE2 (3). However, more studies need to be done as neutralizing antibodies can result in antibody-dependent enhancement, in which the viral entry and replication within the host cell is increased (4). One potential way to combat antibody-dependent enhancement is the use of nanobodies (4). Furthermore, there are currently several vaccine strategies that are in clinical trials, or recently federally approved, that utilize the spike protein in different forms (e.g. full length, S1 RBD, RBD-Fc, N-terminal) for protecting against SARS-CoV-2 infection (4,5). These vaccine strategies include DNA vaccines, viral vector-based vaccines, RNA vaccines, and subunit vaccines (4,5).

References

1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658

2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

3. Ho M. (2020). Perspectives on the development of neutralizing antibodies against SARS-CoV-2. Antibody Therapeutics. https://doi.org/10.1093/abt/tbaa009

4. Samrat, S. K., Tharappel, A. M., Li, Z., & Li, H. (2020). Prospect of SARS-CoV-2 spike protein: Potential role in vaccine and therapeutic development. Virus Research. https://doi.org/10.1016/j.virusres.2020.198141

5. Sternberg, A., & Naujokat, C. (2020). Structural features of coronavirus SARS-CoV-2 spike protein: Targets for vaccination. Life Sciences. https://doi.org/10.1016/j.lfs.2020.118056

Long Name

Spike Protein

Alternate Names

S Protein

Gene Symbol

S

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Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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