SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11931

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

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Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 UK variant (B.1.1.7) Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 UK variant (B.1.1.7) Spike protein.

Specificity

May cross-react with several virus of interest (VOI) variant lineages that contains P681H mutation, including B.11.318, B.1.621, B.1.621.1, P.3. But all of these lineages are rarely present in current pandemic.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK - BSA Free

ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK - BSA Free [NBP3-11931]

ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK - BSA Free [NBP3-11931]

ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - Examination of Alpha Variant Spike Antibody with Spike S1 Protein of SARS-CoV-2 Variants. Coating Antigen: SARS-CoV-2 spike S1 proteins, including WT, alpha variant (B.1.1.7) UK, beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), and mu variant (B.1.621), 1ug/mL, incubate at 4C overnight. Detection Antibodies: SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) UK, dilution: 200-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Spike Antibody Alpha Variant (B.1.1.7) UK can specifically detect alpha variant and Mu spike S1 protein, but not WT and other tested variants.
Western Blot: SARS-CoV-2 Spike AntibodyAlpha Variant, B.1.1.7, UKBSA Free [NBP3-11931]

Western Blot: SARS-CoV-2 Spike AntibodyAlpha Variant, B.1.1.7, UKBSA Free [NBP3-11931]

Western Blot: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - Validation of UK Variant Spike Antibodies with SARS-CoV-2 UK Variant Spike S1 Protein Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and UK variant (B.1.1.7). Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11931, 1 ug/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. SARS-CoV-2 Spike antibody (NBP3-11931) can specifically detect UK variant spike S1 protein, but not WT spike S1 protein.
ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK - BSA Free [NBP3-11931]

ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK - BSA Free [NBP3-11931]

ELISA: SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK [NBP3-11931] - ELISA Validation of UK Variant Spike Antibodies with Mutant and WT Peptide Coating Antigen: SARS-CoV-2 spike S1 peptides, including WT and UK variant (B.1.1.7), 1 ug/mL, incubate at 4 C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, NBP3-11931, dilution: 0.1-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20,000, incubate at RT for 1 hr. Immunogen region of antibody NBP3-11931 includes site 681P that was mutated in B.1.1.7. SARS-CoV-2 Spike antibody (NBP3-11931) can specifically detect UK variant spike S1 peptide (681H), but not WT peptide (681P).
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Applications for SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK - BSA Free

Application
Recommended Usage

Western Blot

1 ug/ml
Application Notes
Antibody can specifically detect SARS-CoV-2 UK Variant (B.1.1.7) Spike S1 protein, but not SARS-CoV-2 WT Spike S1 protein by ELISA. It can also detect mutant peptide, but not WT peptide.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spike

The SARS-CoV-2 Spike protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1,2). The spike protein is the largest of the structural proteins, which also include the membrane (M), envelope (E), and nucleocapsid (N) proteins (1,2). The SARS-CoV-2 spike protein is a 1273 amino acid (aa) heterotrimeric class I fusion protein with each monomer having a theoretical molecular weight of approximately 180 kDa (1). The club-shaped spike protein contains several functional regions and domains including the S1 globular head region which contains the N-terminal receptor-binding domain (RBD) and the S2 stem region that contains the C-terminal fusion domain, two heptad regions, a transmembrane domain, and a cytoplasmic tail (1,2). The viral spike protein is critical for attachment of the virus with the host cell, resulting in fusion and virus entry into the cell (1,2). More specifically, the RBD of the spike protein is responsible for binding to the cell surface receptor angiotensin converting enzyme 2 (ACE2) (1,2). This spike-ACE2 interaction results in a conformational change permitting furin cleavage between the S1 and S2 domains and then cleavage at S2' by TMPRRS2, or another protease, allowing membrane fusion (1,2).

Given the critical role of the spike protein RBD in the interaction with the ACE2 receptor and viral entry, a number of neutralizing antibodies against the RBD have been developed as potential therapeutics for treating COVID-19 (3). These antibodies bind the RBD domain on the S1 subunit inhibiting the interaction with ACE2 (3). However, more studies need to be done as neutralizing antibodies can result in antibody-dependent enhancement, in which the viral entry and replication within the host cell is increased (4). One potential way to combat antibody-dependent enhancement is the use of nanobodies (4). Furthermore, there are currently several vaccine strategies that are in clinical trials, or recently federally approved, that utilize the spike protein in different forms (e.g. full length, S1 RBD, RBD-Fc, N-terminal) for protecting against SARS-CoV-2 infection (4,5). These vaccine strategies include DNA vaccines, viral vector-based vaccines, RNA vaccines, and subunit vaccines (4,5).

References

1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658

2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

3. Ho M. (2020). Perspectives on the development of neutralizing antibodies against SARS-CoV-2. Antibody Therapeutics. https://doi.org/10.1093/abt/tbaa009

4. Samrat, S. K., Tharappel, A. M., Li, Z., & Li, H. (2020). Prospect of SARS-CoV-2 spike protein: Potential role in vaccine and therapeutic development. Virus Research. https://doi.org/10.1016/j.virusres.2020.198141

5. Sternberg, A., & Naujokat, C. (2020). Structural features of coronavirus SARS-CoV-2 spike protein: Targets for vaccination. Life Sciences. https://doi.org/10.1016/j.lfs.2020.118056

Long Name

Spike Protein

Alternate Names

S Protein

Gene Symbol

S

Additional Spike Products

Product Documents for SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 Spike Antibody - Alpha Variant, B.1.1.7, UK - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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