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SARS Spike Protein Antibody (D005) - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90989

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

SARS-CoV, SARS-CoV-2

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Human IgG1 Clone # D005

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for SARS Spike Protein Antibody (D005) - Chimeric

Immunogen

Recombinant SARS-CoV Spike RBD Protein

Reactivity Notes

This antibody has cross-reactivity in ELISA with:
SARS-CoV Spike S1 Protein
SARS-CoV-2 Spike S1 Protein
SARS-CoV-2 Spike RBD Protein

No cross-reactivity in ELISA with:
MERS-CoV Spike S1 Protein
HCoV-HKU1 Spike S1 Protein
HCoV-HKU1 Spike S1 Protein
HCoV-NL63 Spike S1 Protein
HCoV-229E Spike S1 Protein
HCoV-OC43 Spike S1+S2 ECD Protein

Specificity

Has cross-reactivity in ELISA with:SARS-CoV-2 Omicron (B.1.1.529) Spike RBD ProteinSARS-CoV-2 Omicron (B.1.1.529) Spike S1 ProteinSARS-CoV-2 Omicron (B.1.1.529) S1+S2 trimer ProteinSARS-CoV-2 Delta (B.1.617.2) Spike RBD ProteinSARS-CoV-2 Delta (B.1.617.2) Spike S1+S2 ProteinSARS-CoV Spike S1 ProteinSARS-CoV-2 Spike S1 ProteinSARS-CoV-2 Spike RBD ProteinNo cross-reactivity in ELISA with:MERS-CoV Spike S1 ProteinHCoV-HKU1 (isolate N1) Spike S1 ProteinHCoV-HKU1 (isolate N5) Spike S1 ProteinHCoV-NL63 Spike S1 ProteinHCoV-229E Spike S1 ProteinHCoV-OC43 Spike S1+S2 ECD Protein

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This Sars Spike Protein Antibody (D005) is a chimeric monoclonal antibody combining the constant domains of human IgG1 with mouse variable regions.
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Applications for SARS Spike Protein Antibody (D005) - Chimeric

Application
Recommended Usage

ELISA

1:5000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS Spike Protein

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients..

Alternate Names

E2, E2 glycoprotein, Human coronavirus spike glycoprotein, Peplomer protein, S, S glycoprotein, SARS coronavirus S protein, SARS coronavirus Spike Protein, SARS CoV, SARS CoV S glycoprotein, SARS CoV Spike protein, SARSCoV, SARS-CoV S protein, SARS-COV Spike, Severe acute respiratory syndrome spike glycoprotein, Severe acute respiratory syndrome virus spike glycoprotein, Spike glycoprotein, VGL2, SARS-COV-2 S protein, SARS-COV-2 Spike glycoprotein, Spike

Additional SARS Spike Protein Products

Product Documents for SARS Spike Protein Antibody (D005) - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for SARS Spike Protein Antibody (D005) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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