SARS Spike Protein Antibody (D005) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90989CL1

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

SARS-CoV, SARS-CoV-2

Applications

ELISA

Label

CoraFluor 1

Antibody Source

Monoclonal Human IgG1 Clone # D005

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SARS Spike Protein Antibodies »

Product Summary for SARS Spike Protein Antibody (D005) [CoraFluor™ 1]

Immunogen

Recombinant SARS-CoV Spike RBD Protein

Reactivity Notes

This antibody has cross-reactivity in ELISA with:
SARS-CoV Spike S1 Protein
SARS-CoV-2 Spike S1 Protein
SARS-CoV-2 Spike RBD Protein

No cross-reactivity in ELISA with:
MERS-CoV Spike S1 Protein
HCoV-HKU1 Spike S1 Protein
HCoV-HKU1 Spike S1 Protein
HCoV-NL63 Spike S1 Protein
HCoV-229E Spike S1 Protein
HCoV-OC43 Spike S1+S2 ECD Protein

Specificity

Has cross-reactivity in ELISA with:SARS-CoV-2 Omicron (B.1.1.529) Spike RBD ProteinSARS-CoV-2 Omicron (B.1.1.529) Spike S1 ProteinSARS-CoV-2 Omicron (B.1.1.529) S1+S2 trimer ProteinSARS-CoV-2 Delta (B.1.617.2) Spike RBD ProteinSARS-CoV-2 Delta (B.1.617.2) Spike S1+S2 ProteinSARS-CoV Spike S1 ProteinSARS-CoV-2 Spike S1 ProteinSARS-CoV-2 Spike RBD ProteinNo cross-reactivity in ELISA with:MERS-CoV Spike S1 ProteinHCoV-HKU1 (isolate N1) Spike S1 ProteinHCoV-HKU1 (isolate N5) Spike S1 ProteinHCoV-NL63 Spike S1 ProteinHCoV-229E Spike S1 ProteinHCoV-OC43 Spike S1+S2 ECD Protein

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for SARS Spike Protein Antibody (D005) [CoraFluor™ 1]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: SARS Spike Protein

It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients..

Alternate Names

E2, E2 glycoprotein, Human coronavirus spike glycoprotein, Peplomer protein, S, S glycoprotein, SARS coronavirus S protein, SARS coronavirus Spike Protein, SARS CoV, SARS CoV S glycoprotein, SARS CoV Spike protein, SARSCoV, SARS-CoV S protein, SARS-COV Spike, Severe acute respiratory syndrome spike glycoprotein, Severe acute respiratory syndrome virus spike glycoprotein, Spike glycoprotein, VGL2

Additional SARS Spike Protein Products

Product Documents for SARS Spike Protein Antibody (D005) [CoraFluor™ 1]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for SARS Spike Protein Antibody (D005) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.