Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Primate

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all Semaphorin 3B Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human SEMA3B protein sequence (between residues 100-200). [UniProt# Q13214]

Localization

Secreted. Accumulates in the endoplasmic reticulum.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Semaphorin 3B Antibody

Western Blot: Semaphorin 3B Antibody [NB100-2218]

Western Blot: Semaphorin 3B Antibody [NB100-2218]

Western Blot: Semaphorin 3B Antibody [NB100-2218] - Western blot analysis of SEMA3B in A. Hela WCE B. Ntera2 C. A431 D. HepG2 E. MCF7 F. NIH/3T3 G. PC12 H. Cos7
Immunocytochemistry/ Immunofluorescence: Semaphorin 3B Antibody [NB100-2218]

Immunocytochemistry/ Immunofluorescence: Semaphorin 3B Antibody [NB100-2218]

Immunocytochemistry/Immunofluorescence: Semaphorin 3B Antibody [NB100-2218] - SEMA3B antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.
Immunohistochemistry-Paraffin: Semaphorin 3B Antibody [NB100-2218]

Immunohistochemistry-Paraffin: Semaphorin 3B Antibody [NB100-2218]

Immunohistochemistry-Paraffin: Semaphorin 3B Antibody [NB100-2218] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse kidney using 1:200 dilution of rabbit anti-Semaphorin 3B antibody. This antibody generated a weak to moderate cytoplasmic staining in the cells of various renal tubules and glomeruli.
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Applications for Semaphorin 3B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:40

Immunohistochemistry

1:100 - 1:200

Immunohistochemistry-Paraffin

1:100 - 1:200

Simple Western

1:25

Western Blot

1:500-1:2000
Application Notes
This SEMA3B antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry paraffin embedded sections. In Western Blot, a band is seen at ~50 kDa, representing the secreted form of the protein and also a faint band at ~83 kDa, representing the pro-form of the protein. In ICC/IF, strong staining of endoplasmic reticulum and lighter signal in cytoplasm was observed in neuro2a cells. In IHC-P, staining was observed secreted, in the cytoplasm, and in the endoplasmic reticulum of mouse kidney tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Semaphorin 3B

SEMA3B (semaphorin-3B) belong to semaphorins family, a large class of secreted or membrane-associated proteins which act as chemotactic cues for cell migration and axon guidance during CNS development. Secreted SEMA3s are recruited to plexins by neuropilin (NP) receptors and NP1 preferentially binds to SEMA3B. Neuronal and non-neuronal tissues exhibit abundant and differential expression of SEMA3B which inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. Besides CNS, semaphorins are expressed in other tissues also wherein they implicate in organogenesis, cell survival, proliferation, apoptosis, migration, angiogenesis, immuno-regulation and tumorigenesis. SEMA3B and another secreted semaphorin SEMA3F have been isolated from a region on human chromosome 3p21.3 which is commonly found deleted in tumors. SEMA3B is either absent or expressed at very low levels in ovarian, lung, and breast cancer cells, and when its expression is restored, it inhibits tumor growth as well as angiogenesis suggesting its role as tumor suppressor.

Alternate Names

LUCA-1, SEMA3B, SEMA5, SEMAA, SEMAV

Gene Symbol

SEMA3B

Additional Semaphorin 3B Products

Product Documents for Semaphorin 3B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Semaphorin 3B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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