Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI6C2

Concentration

1 mg/ml

View all sFRP-2 Antibodies »

Product Summary for sFRP-2 Antibody (OTI6C2)

Immunogen

Human recombinant protein fragment corresponding to amino acids 25-295 of human SFRP2(NP_003004) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for sFRP-2 Antibody (OTI6C2)

Western Blot: sFRP-2 Antibody (OTI6C2) [NBP2-45804]

Western Blot: sFRP-2 Antibody (OTI6C2) [NBP2-45804]

Western Blot: sFRP-2 Antibody (OTI6C2) [NBP2-45804] - Analysis of extracts (35ug) from 3 different cell lines and 5 different tissue lysates by using anti-SFRP2 monoclonal antibody (1:500).
Immunohistochemistry: sFRP-2 Antibody (OTI6C2) [NBP2-45804]

Immunohistochemistry: sFRP-2 Antibody (OTI6C2) [NBP2-45804]

Immunohistochemistry: sFRP-2 Antibody (OTI6C2) [NBP2-45804] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: sFRP-2 Antibody (OTI6C2) [NBP2-45804]

Immunohistochemistry: sFRP-2 Antibody (OTI6C2) [NBP2-45804]

Immunohistochemistry: sFRP-2 Antibody (OTI6C2) [NBP2-45804] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
View 3 more images

Applications for sFRP-2 Antibody (OTI6C2)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: sFRP-2

SFRP2 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq]

Long Name

Secreted Frizzled Related Protein 2

Alternate Names

SARP-1, SDF-5, sFRP2

Gene Symbol

SFRP2

Additional sFRP-2 Products

Product Documents for sFRP-2 Antibody (OTI6C2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for sFRP-2 Antibody (OTI6C2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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