Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI6C2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all sFRP-2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 25-295 of human SFRP2(NP_003004) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for sFRP-2 Antibody (OTI6C2) - Azide and BSA Free

Western Blot: sFRP-2 Antibody (OTI6C2)Azide and BSA Free [NBP2-74151]

Western Blot: sFRP-2 Antibody (OTI6C2)Azide and BSA Free [NBP2-74151]

Western Blot: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of extracts (35ug) from 3 different cell lines and 5 different tissue lysates by using anti-SFRP2 monoclonal antibody (1:500).
Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]

Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]

Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]

Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]

Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
View 3 more images

Applications for sFRP-2 Antibody (OTI6C2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: sFRP-2

SFRP2 encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq]

Long Name

Secreted Frizzled Related Protein 2

Alternate Names

SARP-1, SDF-5, sFRP2

Gene Symbol

SFRP2

Additional sFRP-2 Products

Product Documents for sFRP-2 Antibody (OTI6C2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for sFRP-2 Antibody (OTI6C2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.