sFRP-2 Antibody (OTI6C2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74151
![Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]](https://resources.bio-techne.com/images/products/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74151-img0002.jpg "Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]")
![Western Blot: sFRP-2 Antibody (OTI6C2)Azide and BSA Free [NBP2-74151]](https://resources.bio-techne.com/images/products/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Western-Blot-NBP2-74151-img0006.jpg "Western Blot: sFRP-2 Antibody (OTI6C2)Azide and BSA Free [NBP2-74151]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI6C2
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary for sFRP-2 Antibody (OTI6C2) - Azide and BSA Free
Immunogen
Human recombinant protein fragment corresponding to amino acids 25-295 of human SFRP2(NP_003004) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for sFRP-2 Antibody (OTI6C2) - Azide and BSA Free
Western Blot: sFRP-2 Antibody (OTI6C2)Azide and BSA Free [NBP2-74151]
Western Blot: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of extracts (35ug) from 3 different cell lines and 5 different tissue lysates by using anti-SFRP2 monoclonal antibody (1:500).![Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]](https://resources.bio-techne.com/images/products/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74151-img0005.jpg "Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]")
Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]
Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]](https://resources.bio-techne.com/images/products/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74151-img0001.jpg "Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]")
Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]
Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]](https://resources.bio-techne.com/images/products/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74151-img0003.jpg "Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]")
![Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]](https://resources.bio-techne.com/images/products/sFRP-2-Antibody-OTI6C2-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74151-img0004.jpg "Immunohistochemistry: sFRP-2 Antibody (OTI6C2) - Azide and BSA Free [NBP2-74151]")
Applications for sFRP-2 Antibody (OTI6C2) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: sFRP-2
Long Name
Secreted Frizzled Related Protein 2
Alternate Names
SARP-1, SDF-5, sFRP2
Gene Symbol
SFRP2
Additional sFRP-2 Products
Product Documents for sFRP-2 Antibody (OTI6C2) - Azide and BSA Free
Product Specific Notices for sFRP-2 Antibody (OTI6C2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...