SHP-1 Antibody (7I1K3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15669

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15669-100ul
NBP3-15669-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7I1K3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SHP-1 Antibodies »

Product Summary for SHP-1 Antibody (7I1K3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 496-595 of human SHP-1 (P29350). SGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHP-1 Antibody (7I1K3)

Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669]

Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669]

Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669] - Western blot analysis of extracts of Raji cells, using SHP-1 antibody (NBP3-15669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry: SHP-1 Antibody (7I1K3) [NBP3-15669]

Immunohistochemistry: SHP-1 Antibody (7I1K3) [NBP3-15669]

Immunohistochemistry: SHP-1 Antibody (7I1K3) [NBP3-15669] - Immunofluorescence analysis of mouse spleen using SHP-1 Rabbit mAb (NBP3-15669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669]

Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669]

Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669] - Western blot analysis of extracts of various cell lines, using SHP-1 antibody (NBP3-15669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
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Applications for SHP-1 Antibody (7I1K3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SHP-1

The SH2 domain-containing phosphatases, SHP-1 and SHP-2, are well-studied cytosolic tyrosine phosphatases that share many structural and regulatory features. They both have two tandem SH2 domains at the N-terminus, followed by a catalytic domain, and an inhibitory C-terminus. Despite their structural similarity, the enzymes appear to have different physiological roles and exhibit different expression patterns. SHP-1 is highly expressed in hematopoietic tissues, and in general has a negative impact on lymphocyte signaling. In contrast, SHP-2 is widely expressed, and in general promotes signaling pathways that lead to differentiation, cell growth, or migration.

Long Name

Src Homology Region 2 Domain Phosphatase 1

Alternate Names

HCPH, PTP1C, PTPN6, SHP1

Gene Symbol

PTPN6

Additional SHP-1 Products

Product Documents for SHP-1 Antibody (7I1K3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHP-1 Antibody (7I1K3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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