SHP-1 Antibody (7I1K3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15669
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 7I1K3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 496-595 of human SHP-1 (P29350). SGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SHP-1 Antibody (7I1K3)
Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669]
Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669] - Western blot analysis of extracts of Raji cells, using SHP-1 antibody (NBP3-15669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.Immunohistochemistry: SHP-1 Antibody (7I1K3) [NBP3-15669]
Immunohistochemistry: SHP-1 Antibody (7I1K3) [NBP3-15669] - Immunofluorescence analysis of mouse spleen using SHP-1 Rabbit mAb (NBP3-15669) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669]
Western Blot: SHP-1 Antibody (7I1K3) [NBP3-15669] - Western blot analysis of extracts of various cell lines, using SHP-1 antibody (NBP3-15669) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.Applications for SHP-1 Antibody (7I1K3)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SHP-1
Long Name
Src Homology Region 2 Domain Phosphatase 1
Alternate Names
HCPH, PTP1C, PTPN6, SHP1
Gene Symbol
PTPN6
Additional SHP-1 Products
Product Documents for SHP-1 Antibody (7I1K3)
Product Specific Notices for SHP-1 Antibody (7I1K3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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