SHP-1 Antibody (M160)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23394

Novus Biologicals, part of Bio-Techne
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NBP3-23394
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M160

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all SHP-1 Antibodies »

Product Specifications

Immunogen

Clone (M160) was generated from a recombinant protein containing amino acids in the C-terminal region of human SHP-1. This sequence is highly conserved in rat and mouse SHP-1. [UniProt# P29350]

Specificity

The antibody detects a 68 kDa protein in human A431 and Jurkat cells, and does not cross-react with SHP2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for SHP-1 Antibody (M160)

SHP-1 Antibody (M160)

Western Blot-SHP-1 Antibody (M160)-NBP3-23394-

Western Blot-SHP-1 Antibody (M160)-NBP3-23394-Analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to lambda phosphatase (lanes 2 & 4) then probed with anti- SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Ser-591) antibody (lanes 3-6). The SHP1 (Ser-591) antibody was used in the presence of phospho-SHP1 (Ser-591) peptide (lane 5) or a non-specific phospho- serine peptide (lane 6).

Applications for SHP-1 Antibody (M160)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SHP-1

The SH2 domain-containing phosphatases, SHP-1 and SHP-2, are well-studied cytosolic tyrosine phosphatases that share many structural and regulatory features. They both have two tandem SH2 domains at the N-terminus, followed by a catalytic domain, and an inhibitory C-terminus. Despite their structural similarity, the enzymes appear to have different physiological roles and exhibit different expression patterns. SHP-1 is highly expressed in hematopoietic tissues, and in general has a negative impact on lymphocyte signaling. In contrast, SHP-2 is widely expressed, and in general promotes signaling pathways that lead to differentiation, cell growth, or migration.

Long Name

Src Homology Region 2 Domain Phosphatase 1

Alternate Names

HCPH, PTP1C, PTPN6, SHP1

Gene Symbol

PTPN6

Additional SHP-1 Products

Product Documents for SHP-1 Antibody (M160)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for SHP-1 Antibody (M160)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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