SHP-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-58968

Novus Biologicals, part of Bio-Techne
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NB100-58968
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SHP-1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KASRTSSKHKEE corresponding to internal region according to NP_002822.2.

Epitope

KASRTSSKHKEE

Specificity

This antibody is expected to recognize both reported isoforms (NP_536858.1and NP_002822.2). Please note this antibody was designed using the mouse sequence, which differs by 1 amino acid from the human sequence.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SHP-1 Antibody

Western Blot: SHP-1 Antibody [NB100-58968]

Western Blot: SHP-1 Antibody [NB100-58968]

Western Blot: SHP-1 Antibody [NB100-58968] - Staining of Mouse Thymus lysate (A) with antibody at 0.01 ug/mL and Rat Thymus lysate with antibody at 0.1 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: SHP-1 Antibody [NB100-58968]

Immunohistochemistry-Paraffin: SHP-1 Antibody [NB100-58968]

Immunohistochemistry-Paraffin: SHP-1 Antibody [NB100-58968] - Staining of paraffin embedded Human Thyroid. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: SHP-1 Antibody [NB100-58968]

Western Blot: SHP-1 Antibody [NB100-58968]

Western Blot: SHP-1 Antibody [NB100-58968] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for SHP-1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.01 - 0.1 ug/mL
Application Notes
Western blot: Approx 70 kDa band observed in Human Liver and Mouse Thymus lysates and approx 70-75 kDa in Rat Thymus lysates (calculated MW of 67.6 kDa according to Human NP_536858.1 and Mouse NP_038573.2, and 69.6kDa according to Rat NP_446360.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SHP-1

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.

Long Name

Src Homology Region 2 Domain Phosphatase 1

Alternate Names

HCPH, PTP1C, PTPN6, SHP1

Entrez Gene IDs

5777 (Human); 15170 (Mouse); 116689 (Rat)

Gene Symbol

PTPN6

UniProt

NP_536858.1 (Human)

Additional SHP-1 Products

Product Documents for SHP-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHP-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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