SHP-1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-58968
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Canine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KASRTSSKHKEE corresponding to internal region according to NP_002822.2.
Epitope
KASRTSSKHKEE
Specificity
This antibody is expected to recognize both reported isoforms (NP_536858.1and NP_002822.2). Please note this antibody was designed using the mouse sequence, which differs by 1 amino acid from the human sequence.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SHP-1 Antibody
Western Blot: SHP-1 Antibody [NB100-58968]
Western Blot: SHP-1 Antibody [NB100-58968] - Staining of Mouse Thymus lysate (A) with antibody at 0.01 ug/mL and Rat Thymus lysate with antibody at 0.1 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.Immunohistochemistry-Paraffin: SHP-1 Antibody [NB100-58968]
Immunohistochemistry-Paraffin: SHP-1 Antibody [NB100-58968] - Staining of paraffin embedded Human Thyroid. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Western Blot: SHP-1 Antibody [NB100-58968]
Western Blot: SHP-1 Antibody [NB100-58968] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.Applications for SHP-1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
5 ug/mL
Peptide ELISA
Detection limit 1:128000
Western Blot
0.01 - 0.1 ug/mL
Application Notes
Western blot: Approx 70 kDa band observed in Human Liver and Mouse Thymus lysates and approx 70-75 kDa in Rat Thymus lysates (calculated MW of 67.6 kDa according to Human NP_536858.1 and Mouse NP_038573.2, and 69.6kDa according to Rat NP_446360.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SHP-1
Long Name
Src Homology Region 2 Domain Phosphatase 1
Alternate Names
HCPH, PTP1C, PTPN6, SHP1
Gene Symbol
PTPN6
UniProt
Additional SHP-1 Products
Product Documents for SHP-1 Antibody
Product Specific Notices for SHP-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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