SHP-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32179

Novus Biologicals, part of Bio-Techne
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NBP1-32179
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all SHP-1 Antibodies »

Product Summary for SHP-1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human SHP-1. The exact sequence is proprietary.

Reactivity Notes

Chicken (80%).

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SHP-1 Antibody

Western Blot: SHP-1 Antibody [NBP1-32179]

Western Blot: SHP-1 Antibody [NBP1-32179]

Western Blot: PTPN6 Antibody [NBP1-32179] - Sample (30 ug of whole cell lysate) A:NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.
Immunoprecipitation: SHP-1 Antibody [NBP1-32179]

Immunoprecipitation: SHP-1 Antibody [NBP1-32179]

Immunoprecipitation: PTPN6 Antibody [NBP1-32179] - HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of SHP1 protein expressing HeLa cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of SHP1 by 3 ug of SHP1 7.5% SDS-PAGE The immunoprecipitated SHP1 protein was detected by SHP1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
SHP-1 Antibody

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] -

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] - SHP-1 antibody detects SHP-1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.SHP-1 stained by SHP-1 antibody (NBP1-32179) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Applications for SHP-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SHP-1

SHP-1 (also called PTP1C, SH-PP1, HCP, SHP and PTPN6) is a protein tyrosine phosphatase (PTP) predominantly expressed in hematopoietic cells, and regulates multiple signaling pathways involved in hematopoietic cell growth, differentiation and activation (1). After cell stimulation, SHP-1 (as well as SHP-2), translocate from cytosol to plasma membrane and bind to the tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). SHP-1 has been involved in the negative regulation of various growth promoting receptors including receptor tyrosine kinase (c-Kit, CSF-1, EGF), cytokine receptors (IL-3, IFN-a/b) and other tyrosine based receptor CD22, B & T cell Ag receptor, Lck, Zap-70 and Vav (3-5).

Long Name

Src Homology Region 2 Domain Phosphatase 1

Alternate Names

HCPH, PTP1C, PTPN6, SHP1

Gene Symbol

PTPN6

Additional SHP-1 Products

Product Documents for SHP-1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHP-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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