SHP-2/PTPN11 Antibody (6D9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37454

Novus Biologicals, part of Bio-Techne
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NBP2-37454-0.025ml
NBP2-37454
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6D9

Format

BSA Free

Concentration

1 mg/ml

View all SHP-2 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human SHP-2/PTPN11 (AA: 263-329) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

68.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SHP-2/PTPN11 Antibody (6D9) - BSA Free

Western Blot: SHP-2/PTPN11 Antibody (6D9)BSA Free [NBP2-37454]

Western Blot: SHP-2/PTPN11 Antibody (6D9)BSA Free [NBP2-37454]

Western Blot: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis using PTPN11 mAb against human PTPN11 recombinant protein. (Expected MW is 33.4 kDa)
Immunocytochemistry/ Immunofluorescence: SHP-2/PTPN11 Antibody (6D9) - BSA Free [NBP2-37454]

Immunocytochemistry/ Immunofluorescence: SHP-2/PTPN11 Antibody (6D9) - BSA Free [NBP2-37454]

Immunocytochemistry/Immunofluorescence: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis of HeLa cells using PTPN11 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody (6D9) - BSA Free [NBP2-37454]

Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody (6D9) - BSA Free [NBP2-37454]

Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody (6D9) [NBP2-37454] - Analysis of breast cancer tissues using PTPN11 mouse mAb with DAB staining.
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Applications for SHP-2/PTPN11 Antibody (6D9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SHP-2

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Long Name

Src Homology Region 2 Domain Phosphatase 2

Alternate Names

PTP1D, PTP2C, PTPN11, SH-PTP2, SHP2

Gene Symbol

PTPN11

Additional SHP-2 Products

Product Documents for SHP-2/PTPN11 Antibody (6D9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHP-2/PTPN11 Antibody (6D9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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