SHP-2/PTPN11 Antibody (OTI3F2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74173

Novus Biologicals, part of Bio-Techne
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NBP2-74173
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3F2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all SHP-2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PTPN11(NP_002825) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

67.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SHP-2/PTPN11 Antibody (OTI3F2) - Azide and BSA Free

Western Blot: SHP-2/PTPN11 Antibody (OTI3F2)Azide and BSA Free [NBP2-74173]

Western Blot: SHP-2/PTPN11 Antibody (OTI3F2)Azide and BSA Free [NBP2-74173]

Western Blot: SHP-2/PTPN11 Antibody (OTI3F2) - Azide and BSA Free [NBP2-74173] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN11/SHP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN11/SHP
Flow Cytometry: SHP-2/PTPN11 Antibody (OTI3F2) - Azide and BSA Free [NBP2-74173]

Flow Cytometry: SHP-2/PTPN11 Antibody (OTI3F2) - Azide and BSA Free [NBP2-74173]

Flow Cytometry: SHP-2/PTPN11 Antibody (OTI3F2) - Azide and BSA Free [NBP2-74173] - Analysis of Jurkat cells, using anti-PTPN11/SHP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: SHP-2/PTPN11 Antibody (OTI3F2)Azide and BSA Free [NBP2-74173]

Western Blot: SHP-2/PTPN11 Antibody (OTI3F2)Azide and BSA Free [NBP2-74173]

Western Blot: SHP-2/PTPN11 Antibody (OTI3F2) - Azide and BSA Free [NBP2-74173] - Analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN11/SHP2 monoclonal antibody.
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Applications for SHP-2/PTPN11 Antibody (OTI3F2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SHP-2

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Long Name

Src Homology Region 2 Domain Phosphatase 2

Alternate Names

PTP1D, PTP2C, PTPN11, SH-PTP2, SHP2

Gene Symbol

PTPN11

Additional SHP-2 Products

Product Documents for SHP-2/PTPN11 Antibody (OTI3F2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHP-2/PTPN11 Antibody (OTI3F2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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