SHP-2/PTPN11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-806

Novus Biologicals, part of Bio-Techne
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NB100-806
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all SHP-2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YENVGLMQQQKSFR corresponding to C-Terminus according to NP_002825.3.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SHP-2/PTPN11 Antibody

Western Blot: SHP-2/PTPN11 Antibody [NB100-806]

Western Blot: SHP-2/PTPN11 Antibody [NB100-806]

Western Blot: SHP-2/PTPN11 Antibody [NB100-806] - Human Muscle lysate (RIPA buffer, 35 ug total protein per lane). Antibody at 2 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: SHP-2/PTPN11 Antibody [NB100-806]

Immunocytochemistry/ Immunofluorescence: SHP-2/PTPN11 Antibody [NB100-806]

Immunocytochemistry/Immunofluorescence: SHP-2/PTPN11 Antibody [NB100-806] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: SHP-2/PTPN11 Antibody [NB100-806]

Flow Cytometry: SHP-2/PTPN11 Antibody [NB100-806]

Flow Cytometry: SHP-2/PTPN11 Antibody [NB100-806] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for SHP-2/PTPN11 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:28000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 70 kDa band observed in human skeletal muscle lysate (calculated MW of 68.0 kDa band according to NP_002825.3).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SHP-2

A protein tyrosine phosphatase that is widely expressed and plays a regulatory role in various cell signaling events that are important for many cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration.

Long Name

Src Homology Region 2 Domain Phosphatase 2

Alternate Names

PTP1D, PTP2C, PTPN11, SH-PTP2, SHP2

Entrez Gene IDs

5781 (Human); 19247 (Mouse); 25622 (Rat)

Gene Symbol

PTPN11

UniProt

NP_002825.3

Additional SHP-2 Products

Product Documents for SHP-2/PTPN11 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for SHP-2/PTPN11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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