Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80948

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 44M12D3

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Siglec-3/CD33 Antibodies »

Product Summary for Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free

Immunogen

Amino acids 42-159 of human CD33 were used as the immunogen for the antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free

Western Blot: Siglec-3/CD33 Antibody (44M12D3)Azide and BSA Free [NBP2-80948]

Western Blot: Siglec-3/CD33 Antibody (44M12D3)Azide and BSA Free [NBP2-80948]

Western Blot: Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free [NBP2-80948] - Analysis of CD33 in A) a partial recombinant protein and B) HCT-116 lysate using CD33 antibody at 2 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.
Immunohistochemistry: Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free [NBP2-80948]

Immunohistochemistry: Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free [NBP2-80948]

Immunohistochemistry: Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free [NBP2-80948] - Human lung tissue stained with CD33 antibody (3 ug/ml), peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining. Image from the standard format of this antibody.

Applications for Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20-1:1000

Immunohistochemistry-Paraffin

3-6 ug/ml

Western Blot

2-5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NBP2-80948 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Siglec-3/CD33

CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function. (1-4)

Long Name

Sialic Acid Binding Ig-like Lectin 3

Alternate Names

CD33, gp67, Siglec3

Gene Symbol

CD33

Additional Siglec-3/CD33 Products

Product Documents for Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Siglec-3/CD33 Antibody (44M12D3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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